1wqy

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(New page: 200px<br /><applet load="1wqy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wqy, resolution 2.00&Aring;" /> '''X-RAY structural ana...)
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[[Image:1wqy.gif|left|200px]]<br /><applet load="1wqy" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1wqy.gif|left|200px]]<br /><applet load="1wqy" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1wqy, resolution 2.00&Aring;" />
caption="1wqy, resolution 2.00&Aring;" />
'''X-RAY structural analysis of B-DNA decamer D(CCATTAATGG)2 crystal grown in D2O solution'''<br />
'''X-RAY structural analysis of B-DNA decamer D(CCATTAATGG)2 crystal grown in D2O solution'''<br />
==Overview==
==Overview==
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It has long been suspected that the structure and function of a DNA duplex, can be strongly dependent on its degree of hydration. By neutron, diffraction experiments, we have succeeded in determining most of the, hydrogen (H) and deuterium (D) atomic positions in the decameric, d(CCATTAATGG)2 duplex. Moreover, the D positions in 27 D2O molecules have, been determined. In particular, the complex water network in the minor, groove has been observed in detail. By a combined structural analysis, using 2.0 A resolution X-ray and 3.0 A resolution neutron data, it is, clear that the spine of hydration is built up, not only by a simple, hexagonal hydration pattern (as reported in earlier X-ray studies), but, also by many other water bridges hydrogen-bonded to the DNA strands. The, complexity of the hydration pattern in the minor groove is derived from an, extraordinary variety of orientations displayed by the water molecules.
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It has long been suspected that the structure and function of a DNA duplex can be strongly dependent on its degree of hydration. By neutron diffraction experiments, we have succeeded in determining most of the hydrogen (H) and deuterium (D) atomic positions in the decameric d(CCATTAATGG)2 duplex. Moreover, the D positions in 27 D2O molecules have been determined. In particular, the complex water network in the minor groove has been observed in detail. By a combined structural analysis using 2.0 A resolution X-ray and 3.0 A resolution neutron data, it is clear that the spine of hydration is built up, not only by a simple hexagonal hydration pattern (as reported in earlier X-ray studies), but also by many other water bridges hydrogen-bonded to the DNA strands. The complexity of the hydration pattern in the minor groove is derived from an extraordinary variety of orientations displayed by the water molecules.
==About this Structure==
==About this Structure==
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1WQY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with DOD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WQY OCA].
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1WQY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WQY OCA].
==Reference==
==Reference==
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[[Category: hydrogen]]
[[Category: hydrogen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:34:00 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:47:15 2008''

Revision as of 13:47, 21 February 2008

Image:1wqy.gif

1wqy, resolution 2.00Å

Drag the structure with the mouse to rotate

X-RAY structural analysis of B-DNA decamer D(CCATTAATGG)2 crystal grown in D2O solution

Overview

It has long been suspected that the structure and function of a DNA duplex can be strongly dependent on its degree of hydration. By neutron diffraction experiments, we have succeeded in determining most of the hydrogen (H) and deuterium (D) atomic positions in the decameric d(CCATTAATGG)2 duplex. Moreover, the D positions in 27 D2O molecules have been determined. In particular, the complex water network in the minor groove has been observed in detail. By a combined structural analysis using 2.0 A resolution X-ray and 3.0 A resolution neutron data, it is clear that the spine of hydration is built up, not only by a simple hexagonal hydration pattern (as reported in earlier X-ray studies), but also by many other water bridges hydrogen-bonded to the DNA strands. The complexity of the hydration pattern in the minor groove is derived from an extraordinary variety of orientations displayed by the water molecules.

About this Structure

1WQY is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Complicated water orientations in the minor groove of the B-DNA decamer d(CCATTAATGG)2 observed by neutron diffraction measurements., Arai S, Chatake T, Ohhara T, Kurihara K, Tanaka I, Suzuki N, Fujimoto Z, Mizuno H, Niimura N, Nucleic Acids Res. 2005 May 24;33(9):3017-24. Print 2005. PMID:15914673

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