1wqz
From Proteopedia
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==Overview== | ==Overview== | ||
| - | It has long been suspected that the structure and function of a DNA duplex | + | It has long been suspected that the structure and function of a DNA duplex can be strongly dependent on its degree of hydration. By neutron diffraction experiments, we have succeeded in determining most of the hydrogen (H) and deuterium (D) atomic positions in the decameric d(CCATTAATGG)2 duplex. Moreover, the D positions in 27 D2O molecules have been determined. In particular, the complex water network in the minor groove has been observed in detail. By a combined structural analysis using 2.0 A resolution X-ray and 3.0 A resolution neutron data, it is clear that the spine of hydration is built up, not only by a simple hexagonal hydration pattern (as reported in earlier X-ray studies), but also by many other water bridges hydrogen-bonded to the DNA strands. The complexity of the hydration pattern in the minor groove is derived from an extraordinary variety of orientations displayed by the water molecules. |
==About this Structure== | ==About this Structure== | ||
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[[Category: neutron diffraction]] | [[Category: neutron diffraction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:47:22 2008'' |
Revision as of 13:47, 21 February 2008
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Complicated water orientations in the minor groove of B-DNA decamer D(CCATTAATGG)2 observed by neutron diffraction measurements
Overview
It has long been suspected that the structure and function of a DNA duplex can be strongly dependent on its degree of hydration. By neutron diffraction experiments, we have succeeded in determining most of the hydrogen (H) and deuterium (D) atomic positions in the decameric d(CCATTAATGG)2 duplex. Moreover, the D positions in 27 D2O molecules have been determined. In particular, the complex water network in the minor groove has been observed in detail. By a combined structural analysis using 2.0 A resolution X-ray and 3.0 A resolution neutron data, it is clear that the spine of hydration is built up, not only by a simple hexagonal hydration pattern (as reported in earlier X-ray studies), but also by many other water bridges hydrogen-bonded to the DNA strands. The complexity of the hydration pattern in the minor groove is derived from an extraordinary variety of orientations displayed by the water molecules.
About this Structure
1WQZ is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Complicated water orientations in the minor groove of the B-DNA decamer d(CCATTAATGG)2 observed by neutron diffraction measurements., Arai S, Chatake T, Ohhara T, Kurihara K, Tanaka I, Suzuki N, Fujimoto Z, Mizuno H, Niimura N, Nucleic Acids Res. 2005 May 24;33(9):3017-24. Print 2005. PMID:15914673
Page seeded by OCA on Thu Feb 21 15:47:22 2008
Categories: Protein complex | Arai, S. | Chatake, T. | Fujimoto, Z. | Kurihara, K. | Mizuno, H. | Niimura, N. | Ohhara, T. | Suzuki, N. | Tanaka, I. | DOD | B-dna | Hydration | Hydrogen | Neutron diffraction
