1wwl
From Proteopedia
(New page: 200px<br /><applet load="1wwl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wwl, resolution 2.50Å" /> '''Crystal structure of...) |
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| - | [[Image:1wwl.gif|left|200px]]<br /><applet load="1wwl" size=" | + | [[Image:1wwl.gif|left|200px]]<br /><applet load="1wwl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wwl, resolution 2.50Å" /> | caption="1wwl, resolution 2.50Å" /> | ||
'''Crystal structure of CD14'''<br /> | '''Crystal structure of CD14'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Lipopolysaccharide, the endotoxin of Gram-negative bacteria, induces | + | Lipopolysaccharide, the endotoxin of Gram-negative bacteria, induces extensive immune responses that can lead to fatal septic shock syndrome. The core receptors recognizing lipopolysaccharide are CD14, TLR4, and MD-2. CD14 binds to lipopolysaccharide and presents it to the TLR4/MD-2 complex, which initiates intracellular signaling. In addition to lipopolysaccharide, CD14 is capable of recognizing a few other microbial and cellular products. Here, we present the first crystal structure of CD14 to 2.5 angstroms resolution. A large hydrophobic pocket was found on the NH2-terminal side of the horseshoe-like structure. Previously identified regions involved in lipopolysaccharide binding map to the rim and bottom of the pocket indicating that the pocket is the main component of the lipopolysaccharide-binding site. Mutations that interfere with lipopolysaccharide signaling but not with lipopolysaccharide binding are also clustered in a separate area near the pocket. Ligand diversity of CD14 could be explained by the generous size of the pocket, the considerable flexibility of the rim of the pocket, and the multiplicity of grooves available for ligand binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1WWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1WWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WWL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Jin, M | + | [[Category: Jin, M S.]] |
| - | [[Category: Kim, J | + | [[Category: Kim, J I.]] |
| - | [[Category: Lee, C | + | [[Category: Lee, C H.]] |
| - | [[Category: Lee, C | + | [[Category: Lee, C J.]] |
[[Category: Lee, H.]] | [[Category: Lee, H.]] | ||
| - | [[Category: Lee, J | + | [[Category: Lee, J O.]] |
| - | [[Category: Paik, S | + | [[Category: Paik, S G.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: cd14]] | [[Category: cd14]] | ||
[[Category: lps]] | [[Category: lps]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:48:49 2008'' |
Revision as of 13:48, 21 February 2008
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Crystal structure of CD14
Overview
Lipopolysaccharide, the endotoxin of Gram-negative bacteria, induces extensive immune responses that can lead to fatal septic shock syndrome. The core receptors recognizing lipopolysaccharide are CD14, TLR4, and MD-2. CD14 binds to lipopolysaccharide and presents it to the TLR4/MD-2 complex, which initiates intracellular signaling. In addition to lipopolysaccharide, CD14 is capable of recognizing a few other microbial and cellular products. Here, we present the first crystal structure of CD14 to 2.5 angstroms resolution. A large hydrophobic pocket was found on the NH2-terminal side of the horseshoe-like structure. Previously identified regions involved in lipopolysaccharide binding map to the rim and bottom of the pocket indicating that the pocket is the main component of the lipopolysaccharide-binding site. Mutations that interfere with lipopolysaccharide signaling but not with lipopolysaccharide binding are also clustered in a separate area near the pocket. Ligand diversity of CD14 could be explained by the generous size of the pocket, the considerable flexibility of the rim of the pocket, and the multiplicity of grooves available for ligand binding.
About this Structure
1WWL is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of CD14 and its implications for lipopolysaccharide signaling., Kim JI, Lee CJ, Jin MS, Lee CH, Paik SG, Lee H, Lee JO, J Biol Chem. 2005 Mar 25;280(12):11347-51. Epub 2005 Jan 10. PMID:15644310
Page seeded by OCA on Thu Feb 21 15:48:49 2008
Categories: Mus musculus | Single protein | Jin, M S. | Kim, J I. | Lee, C H. | Lee, C J. | Lee, H. | Lee, J O. | Paik, S G. | NAG | Cd14 | Lps
