1x02
From Proteopedia
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'''Solution structure of stereo array isotope labeled (SAIL) calmodulin'''<br /> | '''Solution structure of stereo array isotope labeled (SAIL) calmodulin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nuclear-magnetic-resonance spectroscopy can determine the | + | Nuclear-magnetic-resonance spectroscopy can determine the three-dimensional structure of proteins in solution. However, its potential has been limited by the difficulty of interpreting NMR spectra in the presence of broadened and overlapping resonance lines and low signal-to-noise ratios. Here we present stereo-array isotope labelling (SAIL), a technique that can overcome many of these problems by applying a complete stereospecific and regiospecific pattern of stable isotopes that is optimal with regard to the quality and information content of the resulting NMR spectra. SAIL uses exclusively chemically and enzymatically synthesized amino acids for cell-free protein expression. We demonstrate for the 17-kDa protein calmodulin and the 41-kDa maltodextrin-binding protein that SAIL offers sharpened lines, spectral simplification without loss of information, and the ability to rapidly collect the structural restraints required to solve a high-quality solution structure for proteins twice as large as commonly solved by NMR. It thus makes a large class of proteins newly accessible to detailed solution structure determination. |
==About this Structure== | ==About this Structure== | ||
| - | 1X02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1X02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X02 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Guntert, P.]] | [[Category: Guntert, P.]] | ||
[[Category: Kainosho, M.]] | [[Category: Kainosho, M.]] | ||
| - | [[Category: Ono, A | + | [[Category: Ono, A M.]] |
[[Category: Terauchi, T.]] | [[Category: Terauchi, T.]] | ||
[[Category: Torizawa, T.]] | [[Category: Torizawa, T.]] | ||
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[[Category: stereo array isotope labeling]] | [[Category: stereo array isotope labeling]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:49:47 2008'' |
Revision as of 13:49, 21 February 2008
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Solution structure of stereo array isotope labeled (SAIL) calmodulin
Overview
Nuclear-magnetic-resonance spectroscopy can determine the three-dimensional structure of proteins in solution. However, its potential has been limited by the difficulty of interpreting NMR spectra in the presence of broadened and overlapping resonance lines and low signal-to-noise ratios. Here we present stereo-array isotope labelling (SAIL), a technique that can overcome many of these problems by applying a complete stereospecific and regiospecific pattern of stable isotopes that is optimal with regard to the quality and information content of the resulting NMR spectra. SAIL uses exclusively chemically and enzymatically synthesized amino acids for cell-free protein expression. We demonstrate for the 17-kDa protein calmodulin and the 41-kDa maltodextrin-binding protein that SAIL offers sharpened lines, spectral simplification without loss of information, and the ability to rapidly collect the structural restraints required to solve a high-quality solution structure for proteins twice as large as commonly solved by NMR. It thus makes a large class of proteins newly accessible to detailed solution structure determination.
About this Structure
1X02 is a Single protein structure of sequence from Xenopus laevis with as ligand. Full crystallographic information is available from OCA.
Reference
Optimal isotope labelling for NMR protein structure determinations., Kainosho M, Torizawa T, Iwashita Y, Terauchi T, Mei Ono A, Guntert P, Nature. 2006 Mar 2;440(7080):52-7. PMID:16511487
Page seeded by OCA on Thu Feb 21 15:49:47 2008
