1x77
From Proteopedia
(New page: 200px<br /><applet load="1x77" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x77, resolution 2.70Å" /> '''Crystal struture of ...) |
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| - | [[Image:1x77.gif|left|200px]]<br /><applet load="1x77" size=" | + | [[Image:1x77.gif|left|200px]]<br /><applet load="1x77" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1x77, resolution 2.70Å" /> | caption="1x77, resolution 2.70Å" /> | ||
'''Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN'''<br /> | '''Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The availability of high-intensity synchrotron facilities, technological | + | The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays. |
==About this Structure== | ==About this Structure== | ||
| - | 1X77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1X77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Agarwal, R.]] | [[Category: Agarwal, R.]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: NYSGXRC, New | + | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] |
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: FMN]] | [[Category: FMN]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:51:47 2008'' |
Revision as of 13:51, 21 February 2008
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Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN
Overview
The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.
About this Structure
1X77 is a Single protein structure of sequence from Pseudomonas aeruginosa pao1 with as ligand. Full crystallographic information is available from OCA.
Reference
Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal., Agarwal R, Bonanno JB, Burley SK, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:16552139
Page seeded by OCA on Thu Feb 21 15:51:47 2008
Categories: Pseudomonas aeruginosa pao1 | Single protein | Agarwal, R. | Burley, S K. | NYSGXRC, New York Structural GenomiX Research Consortium. | Swaminathan, S. | FMN | Fmn bound | Fmn reductase | New york structural genomix research consortium | Nysgxrc | Protein structure initiative (psi) | Structural genomics
