1x8w
From Proteopedia
(New page: 200px<br /><applet load="1x8w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x8w, resolution 3.8Å" /> '''Structure of the Tetr...) |
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| - | [[Image:1x8w.gif|left|200px]]<br /><applet load="1x8w" size=" | + | [[Image:1x8w.gif|left|200px]]<br /><applet load="1x8w" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1x8w, resolution 3.8Å" /> | caption="1x8w, resolution 3.8Å" /> | ||
'''Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site'''<br /> | '''Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Tetrahymena intron is an RNA catalyst, or ribozyme. As part of its | + | The Tetrahymena intron is an RNA catalyst, or ribozyme. As part of its self-splicing reaction, this ribozyme catalyzes phosphoryl transfer between guanosine and a substrate RNA strand. Here we report the refined crystal structure of an active Tetrahymena ribozyme in the absence of its RNA substrate at 3.8 A resolution. The 3'-terminal guanosine (omegaG), which serves as the attacking group for RNA cleavage, forms a coplanar base triple with the G264-C311 base pair, and this base triple is sandwiched by three other base triples. In addition, a metal ion is present in the active site, contacting or positioned close to the ribose of the omegaG and five phosphates. All of these phosphates have been shown to be important for catalysis. Therefore, we provide a picture of how the ribozyme active site positions both a catalytic metal ion and the nucleophilic guanosine for catalysis prior to binding its RNA substrate. |
==About this Structure== | ==About this Structure== | ||
| - | 1X8W is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1X8W is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8W OCA]. |
==Reference== | ==Reference== | ||
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15525509 15525509] | Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15525509 15525509] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cech, T | + | [[Category: Cech, T R.]] |
| - | [[Category: Gooding, A | + | [[Category: Gooding, A R.]] |
[[Category: Guo, F.]] | [[Category: Guo, F.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: ribozyme]] | [[Category: ribozyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:12 2008'' |
Revision as of 13:52, 21 February 2008
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Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site
Overview
The Tetrahymena intron is an RNA catalyst, or ribozyme. As part of its self-splicing reaction, this ribozyme catalyzes phosphoryl transfer between guanosine and a substrate RNA strand. Here we report the refined crystal structure of an active Tetrahymena ribozyme in the absence of its RNA substrate at 3.8 A resolution. The 3'-terminal guanosine (omegaG), which serves as the attacking group for RNA cleavage, forms a coplanar base triple with the G264-C311 base pair, and this base triple is sandwiched by three other base triples. In addition, a metal ion is present in the active site, contacting or positioned close to the ribose of the omegaG and five phosphates. All of these phosphates have been shown to be important for catalysis. Therefore, we provide a picture of how the ribozyme active site positions both a catalytic metal ion and the nucleophilic guanosine for catalysis prior to binding its RNA substrate.
About this Structure
1X8W is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:15525509
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