1x8s
From Proteopedia
(New page: 200px<br /><applet load="1x8s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x8s, resolution 2.503Å" /> '''Structure of the Pa...) |
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| - | [[Image:1x8s.jpg|left|200px]]<br /><applet load="1x8s" size=" | + | [[Image:1x8s.jpg|left|200px]]<br /><applet load="1x8s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1x8s, resolution 2.503Å" /> | caption="1x8s, resolution 2.503Å" /> | ||
'''Structure of the Par-6 PDZ domain with a Pals1 internal ligand'''<br /> | '''Structure of the Par-6 PDZ domain with a Pals1 internal ligand'''<br /> | ||
==Overview== | ==Overview== | ||
| - | PDZ protein interaction domains are typically selective for C-terminal | + | PDZ protein interaction domains are typically selective for C-terminal ligands, but non-C-terminal, 'internal' ligands have also been identified. The PDZ domain from the cell polarity protein Par-6 binds C-terminal ligands and an internal sequence from the protein Pals1/Stardust. The structure of the Pals1-Par-6 PDZ complex reveals that the PDZ ligand-binding site is deformed to allow for internal binding. Whereas binding of the Rho GTPase Cdc42 to a CRIB domain adjacent to the Par-6 PDZ regulates binding of C-terminal ligands, the conformational change that occurs upon binding of Pals1 renders its binding independent of Cdc42. These results suggest a mechanism by which the requirement for a C terminus can be readily bypassed by PDZ ligands and reveal a complex set of cooperative and competitive interactions in Par-6 that are likely to be important for cell polarity regulation. |
==About this Structure== | ==About this Structure== | ||
| - | 1X8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1X8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: DiVittorio, H | + | [[Category: DiVittorio, H M.]] |
| - | [[Category: Penkert, R | + | [[Category: Penkert, R R.]] |
| - | [[Category: Prehoda, K | + | [[Category: Prehoda, K E.]] |
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
[[Category: par-6]] | [[Category: par-6]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:14 2008'' |
Revision as of 13:52, 21 February 2008
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Structure of the Par-6 PDZ domain with a Pals1 internal ligand
Overview
PDZ protein interaction domains are typically selective for C-terminal ligands, but non-C-terminal, 'internal' ligands have also been identified. The PDZ domain from the cell polarity protein Par-6 binds C-terminal ligands and an internal sequence from the protein Pals1/Stardust. The structure of the Pals1-Par-6 PDZ complex reveals that the PDZ ligand-binding site is deformed to allow for internal binding. Whereas binding of the Rho GTPase Cdc42 to a CRIB domain adjacent to the Par-6 PDZ regulates binding of C-terminal ligands, the conformational change that occurs upon binding of Pals1 renders its binding independent of Cdc42. These results suggest a mechanism by which the requirement for a C terminus can be readily bypassed by PDZ ligands and reveal a complex set of cooperative and competitive interactions in Par-6 that are likely to be important for cell polarity regulation.
About this Structure
1X8S is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Internal recognition through PDZ domain plasticity in the Par-6-Pals1 complex., Penkert RR, DiVittorio HM, Prehoda KE, Nat Struct Mol Biol. 2004 Nov;11(11):1122-7. Epub 2004 Oct 10. PMID:15475968
Page seeded by OCA on Thu Feb 21 15:52:14 2008
