1x9l
From Proteopedia
(New page: 200px<br /><applet load="1x9l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x9l" /> '''Solution structure of CuI-DR1885 from Deinoc...) |
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| - | [[Image:1x9l.gif|left|200px]]<br /><applet load="1x9l" size=" | + | [[Image:1x9l.gif|left|200px]]<br /><applet load="1x9l" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1x9l" /> | caption="1x9l" /> | ||
'''Solution structure of CuI-DR1885 from Deinococcus Radiodurans'''<br /> | '''Solution structure of CuI-DR1885 from Deinococcus Radiodurans'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sco1 and Cox17 are accessory proteins required for the correct assembly of | + | Sco1 and Cox17 are accessory proteins required for the correct assembly of eukaryotic cytochrome c oxidase. At variance with Sco1, Cox17 orthologs are found only in eukaryotes. We browsed bacterial genomes to search proteins functionally equivalent to Cox17, and we identified a class of proteins of unknown function displaying a conserved gene neighborhood to bacterial Sco1 genes, all sharing a potential metal binding motif H(M)X10MX21HXM. Two members of this group, DR1885 from Deinococcus radiodurans and CC3502 from Caulobacter crescentus, were expressed, and their interaction with copper was investigated. The solution structure and extended x-ray absorption fine structure data on the former protein reveal that the protein binds copper(I) through a histidine and three Mets in a cupredoxin-like fold. The surface location of the copper-binding site as well as the type of coordination are well poised for metal transfer chemistry, suggesting that DR1885 might transfer copper, taking the role of Cox17 in bacteria. On the basis of our results, a possible pathway for copper delivery to the Cu(A) center in bacteria is proposed. |
==About this Structure== | ==About this Structure== | ||
| - | 1X9L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans_r1 Deinococcus radiodurans r1] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1X9L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans_r1 Deinococcus radiodurans r1] with <scene name='pdbligand=CU1:'>CU1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X9L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Katsaros, N.]] | [[Category: Katsaros, N.]] | ||
[[Category: Kubicek, K.]] | [[Category: Kubicek, K.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
[[Category: CU1]] | [[Category: CU1]] | ||
[[Category: copper binding protein]] | [[Category: copper binding protein]] | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:34 2008'' |
Revision as of 13:52, 21 February 2008
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Solution structure of CuI-DR1885 from Deinococcus Radiodurans
Overview
Sco1 and Cox17 are accessory proteins required for the correct assembly of eukaryotic cytochrome c oxidase. At variance with Sco1, Cox17 orthologs are found only in eukaryotes. We browsed bacterial genomes to search proteins functionally equivalent to Cox17, and we identified a class of proteins of unknown function displaying a conserved gene neighborhood to bacterial Sco1 genes, all sharing a potential metal binding motif H(M)X10MX21HXM. Two members of this group, DR1885 from Deinococcus radiodurans and CC3502 from Caulobacter crescentus, were expressed, and their interaction with copper was investigated. The solution structure and extended x-ray absorption fine structure data on the former protein reveal that the protein binds copper(I) through a histidine and three Mets in a cupredoxin-like fold. The surface location of the copper-binding site as well as the type of coordination are well poised for metal transfer chemistry, suggesting that DR1885 might transfer copper, taking the role of Cox17 in bacteria. On the basis of our results, a possible pathway for copper delivery to the Cu(A) center in bacteria is proposed.
About this Structure
1X9L is a Single protein structure of sequence from Deinococcus radiodurans r1 with as ligand. Full crystallographic information is available from OCA.
Reference
A copper(I) protein possibly involved in the assembly of CuA center of bacterial cytochrome c oxidase., Banci L, Bertini I, Ciofi-Baffoni S, Katsari E, Katsaros N, Kubicek K, Mangani S, Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):3994-9. Epub 2005 Mar 7. PMID:15753304
Page seeded by OCA on Thu Feb 21 15:52:34 2008
Categories: Deinococcus radiodurans r1 | Single protein | Banci, L. | Bertini, I. | Ciofi-Baffoni, S. | Katsari, E. | Katsaros, N. | Kubicek, K. | SPINE, Structural Proteomics in Europe. | CU1 | Copper binding protein | Deinococcus radiodurans | Holo-form | Nmr | Solution structure | Spine | Structural genomics | Structural proteomics in europe
