1xak
From Proteopedia
(New page: 200px<br /><applet load="1xak" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xak, resolution 1.80Å" /> '''STRUCTURE OF THE SAR...) |
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| - | [[Image:1xak.jpg|left|200px]]<br /><applet load="1xak" size=" | + | [[Image:1xak.jpg|left|200px]]<br /><applet load="1xak" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xak, resolution 1.80Å" /> | caption="1xak, resolution 1.80Å" /> | ||
'''STRUCTURE OF THE SARS-CORONAVIRUS ORF7A ACCESSORY PROTEIN'''<br /> | '''STRUCTURE OF THE SARS-CORONAVIRUS ORF7A ACCESSORY PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The open reading frame (ORF) 7a of the SARS-associated coronavirus | + | The open reading frame (ORF) 7a of the SARS-associated coronavirus (SARS-CoV) encodes a unique type I transmembrane protein of unknown function. We have determined the 1.8 A resolution crystal structure of the N-terminal ectodomain of orf7a, revealing a compact seven-stranded beta sandwich unexpectedly similar in fold and topology to members of the Ig superfamily. We also demonstrate that, in SARS-CoV- infected cells, the orf7a protein is expressed and retained intracellularly. Confocal microscopy studies using orf7a and orf7a/CD4 chimeras implicate the short cytoplasmic tail and transmembrane domain in trafficking of the protein within the endoplasmic reticulum and Golgi network. Taken together, our findings provide a structural and cellular framework in which to explore the role of orf7a in SARS-CoV pathogenesis. |
==About this Structure== | ==About this Structure== | ||
| - | 1XAK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http:// | + | 1XAK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Human sars coronavirus]] | [[Category: Human sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Fremont, D | + | [[Category: Fremont, D H.]] |
| - | [[Category: Lee, C | + | [[Category: Lee, C A.]] |
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
| - | [[Category: Nelson, C | + | [[Category: Nelson, C A.]] |
[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
[[Category: i-set ig domain]] | [[Category: i-set ig domain]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:46 2008'' |
Revision as of 13:52, 21 February 2008
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STRUCTURE OF THE SARS-CORONAVIRUS ORF7A ACCESSORY PROTEIN
Overview
The open reading frame (ORF) 7a of the SARS-associated coronavirus (SARS-CoV) encodes a unique type I transmembrane protein of unknown function. We have determined the 1.8 A resolution crystal structure of the N-terminal ectodomain of orf7a, revealing a compact seven-stranded beta sandwich unexpectedly similar in fold and topology to members of the Ig superfamily. We also demonstrate that, in SARS-CoV- infected cells, the orf7a protein is expressed and retained intracellularly. Confocal microscopy studies using orf7a and orf7a/CD4 chimeras implicate the short cytoplasmic tail and transmembrane domain in trafficking of the protein within the endoplasmic reticulum and Golgi network. Taken together, our findings provide a structural and cellular framework in which to explore the role of orf7a in SARS-CoV pathogenesis.
About this Structure
1XAK is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.
Reference
Structure and intracellular targeting of the SARS-coronavirus Orf7a accessory protein., Nelson CA, Pekosz A, Lee CA, Diamond MS, Fremont DH, Structure. 2005 Jan;13(1):75-85. PMID:15642263
Page seeded by OCA on Thu Feb 21 15:52:46 2008
