1xco
From Proteopedia
(New page: 200px<br /><applet load="1xco" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xco, resolution 2.85Å" /> '''Crystal Structure of...) |
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| - | [[Image:1xco.jpg|left|200px]]<br /><applet load="1xco" size=" | + | [[Image:1xco.jpg|left|200px]]<br /><applet load="1xco" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xco, resolution 2.85Å" /> | caption="1xco, resolution 2.85Å" /> | ||
'''Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate'''<br /> | '''Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Phosphotransacetylase (Pta) [EC 2.3.1.8] plays a major role in acetate | + | Phosphotransacetylase (Pta) [EC 2.3.1.8] plays a major role in acetate metabolism by catalyzing the reversible transfer of the acetyl group between coenzyme A (CoA) and orthophosphate: CH(3)COSCoA+HPO(4)(2-)<-->CH(3)COOPO(3)(2-) +CoASH. In this study, we report the crystal structures of Pta from Bacillus subtilis at 2.75 A resolution and its complex with acetyl phosphate, one of its substrates, at 2.85 A resolution. In addition, the Pta activity of the enzyme has been assayed. The enzyme folds into an alpha/beta architecture with two domains separated by a prominent cleft, very similar to two other known Pta structures. The enzyme-acetyl phosphate complex structure reveals a few potential substrate binding sites. Two of them are located in the middle of the interdomain cleft: each one is surrounded by a region of strictly and highly conserved residues. High structural similarities are found with 4-hydroxythreonine-4-phosphate dehydrogenase (PdxA), and isocitrate and isopropylmalate dehydrogenases, all of which utilize NADP+ as their cofactor, which binds in the interdomain cleft. Their substrate binding sites are close to the acetyl phosphate binding sites of Pta in the cleft as well. These results suggest that the CoA is likely to bind to the interdomain cleft of Pta in a similar way as NADP+ binds to the other three enzymes. |
==About this Structure== | ==About this Structure== | ||
| - | 1XCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with SO4 and UVW as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphate_acetyltransferase Phosphate acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.8 2.3.1.8] Full crystallographic information is available from [http:// | + | 1XCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=UVW:'>UVW</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphate_acetyltransferase Phosphate acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.8 2.3.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Adams, P.]] | [[Category: Adams, P.]] | ||
| - | [[Category: BSGC, Berkeley | + | [[Category: BSGC, Berkeley Structural Genomics Center.]] |
[[Category: Jancarik, J.]] | [[Category: Jancarik, J.]] | ||
[[Category: Kim, R.]] | [[Category: Kim, R.]] | ||
| - | [[Category: Kim, S | + | [[Category: Kim, S H.]] |
[[Category: Lou, Y.]] | [[Category: Lou, Y.]] | ||
| - | [[Category: Xu, Q | + | [[Category: Xu, Q S.]] |
[[Category: Yokota, H.]] | [[Category: Yokota, H.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:53:20 2008'' |
Revision as of 13:53, 21 February 2008
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Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate
Overview
Phosphotransacetylase (Pta) [EC 2.3.1.8] plays a major role in acetate metabolism by catalyzing the reversible transfer of the acetyl group between coenzyme A (CoA) and orthophosphate: CH(3)COSCoA+HPO(4)(2-)<-->CH(3)COOPO(3)(2-) +CoASH. In this study, we report the crystal structures of Pta from Bacillus subtilis at 2.75 A resolution and its complex with acetyl phosphate, one of its substrates, at 2.85 A resolution. In addition, the Pta activity of the enzyme has been assayed. The enzyme folds into an alpha/beta architecture with two domains separated by a prominent cleft, very similar to two other known Pta structures. The enzyme-acetyl phosphate complex structure reveals a few potential substrate binding sites. Two of them are located in the middle of the interdomain cleft: each one is surrounded by a region of strictly and highly conserved residues. High structural similarities are found with 4-hydroxythreonine-4-phosphate dehydrogenase (PdxA), and isocitrate and isopropylmalate dehydrogenases, all of which utilize NADP+ as their cofactor, which binds in the interdomain cleft. Their substrate binding sites are close to the acetyl phosphate binding sites of Pta in the cleft as well. These results suggest that the CoA is likely to bind to the interdomain cleft of Pta in a similar way as NADP+ binds to the other three enzymes.
About this Structure
1XCO is a Single protein structure of sequence from Bacillus subtilis with and as ligands. Active as Phosphate acetyltransferase, with EC number 2.3.1.8 Full crystallographic information is available from OCA.
Reference
Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate., Xu QS, Jancarik J, Lou Y, Kuznetsova K, Yakunin AF, Yokota H, Adams P, Kim R, Kim SH, J Struct Funct Genomics. 2005 Dec;6(4):269-79. Epub 2005 Nov 9. PMID:16283428
Page seeded by OCA on Thu Feb 21 15:53:20 2008
Categories: Bacillus subtilis | Phosphate acetyltransferase | Single protein | Adams, P. | BSGC, Berkeley Structural Genomics Center. | Jancarik, J. | Kim, R. | Kim, S H. | Lou, Y. | Xu, Q S. | Yokota, H. | SO4 | UVW | Berkeley structural genomics center | Bsgc | Bsgc structure funded by nih | Protein structure initiative | Psi | Structural genomics
