1xe8
From Proteopedia
(New page: 200px<br /><applet load="1xe8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xe8, resolution 2.8Å" /> '''Crystal structure of ...) |
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| - | [[Image:1xe8.gif|left|200px]]<br /><applet load="1xe8" size=" | + | [[Image:1xe8.gif|left|200px]]<br /><applet load="1xe8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xe8, resolution 2.8Å" /> | caption="1xe8, resolution 2.8Å" /> | ||
'''Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly roll fold.'''<br /> | '''Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly roll fold.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We determined the three-dimensional crystal structure of the protein | + | We determined the three-dimensional crystal structure of the protein YML079wp, encoded by a hypothetical open reading frame from Saccharomyces cerevisiae to a resolution of 1.75 A. The protein has no close homologs and its molecular and cellular functions are unknown. The structure of the protein is a jelly-roll fold consisting of ten beta-strands organized in two parallel packed beta-sheets. The protein has strong structural resemblance to the plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) but also to some plant and bacterial enzymes (epimerase, germin). The protein forms homodimers in the crystal, confirming measurements of its molecular mass in solution. Two monomers have their beta-sheet packed together to form the dimer. The presence of a hydrophobic ligand in a well conserved pocket inside the barrel and local sequence similarity with bacterial epimerases may suggest a biochemical function for this protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1XE8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ADE, CIT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1XE8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ADE:'>ADE</scene>, <scene name='pdbligand=CIT:'>CIT</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XE8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Meyer, P.]] | [[Category: Meyer, P.]] | ||
[[Category: Quevillon-Cheruel, S.]] | [[Category: Quevillon-Cheruel, S.]] | ||
| - | [[Category: Sierra-Gallay, I | + | [[Category: Sierra-Gallay, I Li de La.]] |
[[Category: Sorel, I.]] | [[Category: Sorel, I.]] | ||
| - | [[Category: Tilbeurgh, H | + | [[Category: Tilbeurgh, H Van.]] |
| - | [[Category: Zhou, C | + | [[Category: Zhou, C Z.]] |
[[Category: ADE]] | [[Category: ADE]] | ||
[[Category: CIT]] | [[Category: CIT]] | ||
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[[Category: yml079wp]] | [[Category: yml079wp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:53:56 2008'' |
Revision as of 13:53, 21 February 2008
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Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly roll fold.
Overview
We determined the three-dimensional crystal structure of the protein YML079wp, encoded by a hypothetical open reading frame from Saccharomyces cerevisiae to a resolution of 1.75 A. The protein has no close homologs and its molecular and cellular functions are unknown. The structure of the protein is a jelly-roll fold consisting of ten beta-strands organized in two parallel packed beta-sheets. The protein has strong structural resemblance to the plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) but also to some plant and bacterial enzymes (epimerase, germin). The protein forms homodimers in the crystal, confirming measurements of its molecular mass in solution. Two monomers have their beta-sheet packed together to form the dimer. The presence of a hydrophobic ligand in a well conserved pocket inside the barrel and local sequence similarity with bacterial epimerases may suggest a biochemical function for this protein.
About this Structure
1XE8 is a Single protein structure of sequence from Saccharomyces cerevisiae with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly-roll fold., Zhou CZ, Meyer P, Quevillon-Cheruel S, De La Sierra-Gallay IL, Collinet B, Graille M, Blondeau K, Francois JM, Leulliot N, Sorel I, Poupon A, Janin J, Van Tilbeurgh H, Protein Sci. 2005 Jan;14(1):209-15. PMID:15608122
Page seeded by OCA on Thu Feb 21 15:53:56 2008
Categories: Saccharomyces cerevisiae | Single protein | Collinet, B. | Graille, M. | Janin, J. | Leulliot, N. | Meyer, P. | Quevillon-Cheruel, S. | Sierra-Gallay, I Li de La. | Sorel, I. | Tilbeurgh, H Van. | Zhou, C Z. | ADE | CIT | GOL | Cupin superfamily | Jelly roll motif | S. cerevisiae | Structural genomics | Yml079wp
