1xiw
From Proteopedia
(New page: 200px<br /> <applet load="1xiw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xiw, resolution 1.90Å" /> '''Crystal structure o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1xiw.gif|left|200px]]<br /> | + | [[Image:1xiw.gif|left|200px]]<br /><applet load="1xiw" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1xiw" size=" | + | |
caption="1xiw, resolution 1.90Å" /> | caption="1xiw, resolution 1.90Å" /> | ||
'''Crystal structure of human CD3-e/d dimer in complex with a UCHT1 single-chain antibody fragment'''<br /> | '''Crystal structure of human CD3-e/d dimer in complex with a UCHT1 single-chain antibody fragment'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The alpha/beta T cell receptor complex transmits signals from MHC/peptide | + | The alpha/beta T cell receptor complex transmits signals from MHC/peptide antigens through a set of constitutively associated signaling molecules, including CD3-epsilon/gamma and CD3-epsilon/delta. We report the crystal structure at 1.9-A resolution of a complex between a human CD3-epsilon/delta ectodomain heterodimer and a single-chain fragment of the UCHT1 antibody. CD3-epsilon/delta and CD3-epsilon/gamma share a conserved interface between the Ig-fold ectodomains, with parallel packing of the two G strands. CD3-delta has a more electronegative surface and a more compact Ig fold than CD3-gamma; thus, the two CD3 heterodimers have distinctly different molecular surfaces. The UCHT1 antibody binds near an acidic region of CD3-epsilon opposite the dimer interface, occluding this region from direct interaction with the TCR. This immunodominant epitope may be a uniquely accessible surface in the TCR/CD3 complex, because there is overlap between the binding site of the UCHT1 and OKT3 antibodies. Determination of the CD3-epsilon/delta structure completes the set of TCR/CD3 globular ectodomains and contributes information about exposed CD3 surfaces. |
| + | |||
| + | ==Disease== | ||
| + | Known diseases associated with this structure: Immunodeficiency due to defect in CD3-epsilon OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=186830 186830]], Severe combined immunodeficiency, T-negative/B-positive type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=186790 186790]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 1XIW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1XIW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XIW OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 17: | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Arnett, K | + | [[Category: Arnett, K L.]] |
| - | [[Category: Harrison, S | + | [[Category: Harrison, S C.]] |
| - | [[Category: Wiley, D | + | [[Category: Wiley, D C.]] |
[[Category: antibody-antigen complex]] | [[Category: antibody-antigen complex]] | ||
[[Category: cd3-delta]] | [[Category: cd3-delta]] | ||
| Line 24: | Line 26: | ||
[[Category: ucht1-scfv]] | [[Category: ucht1-scfv]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:55:15 2008'' |
Revision as of 13:55, 21 February 2008
|
Crystal structure of human CD3-e/d dimer in complex with a UCHT1 single-chain antibody fragment
Contents |
Overview
The alpha/beta T cell receptor complex transmits signals from MHC/peptide antigens through a set of constitutively associated signaling molecules, including CD3-epsilon/gamma and CD3-epsilon/delta. We report the crystal structure at 1.9-A resolution of a complex between a human CD3-epsilon/delta ectodomain heterodimer and a single-chain fragment of the UCHT1 antibody. CD3-epsilon/delta and CD3-epsilon/gamma share a conserved interface between the Ig-fold ectodomains, with parallel packing of the two G strands. CD3-delta has a more electronegative surface and a more compact Ig fold than CD3-gamma; thus, the two CD3 heterodimers have distinctly different molecular surfaces. The UCHT1 antibody binds near an acidic region of CD3-epsilon opposite the dimer interface, occluding this region from direct interaction with the TCR. This immunodominant epitope may be a uniquely accessible surface in the TCR/CD3 complex, because there is overlap between the binding site of the UCHT1 and OKT3 antibodies. Determination of the CD3-epsilon/delta structure completes the set of TCR/CD3 globular ectodomains and contributes information about exposed CD3 surfaces.
Disease
Known diseases associated with this structure: Immunodeficiency due to defect in CD3-epsilon OMIM:[186830], Severe combined immunodeficiency, T-negative/B-positive type OMIM:[186790]
About this Structure
1XIW is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment., Arnett KL, Harrison SC, Wiley DC, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16268-73. Epub 2004 Nov 8. PMID:15534202
Page seeded by OCA on Thu Feb 21 15:55:15 2008
