1xkw
From Proteopedia
(New page: 200px<br /><applet load="1xkw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xkw, resolution 2.0Å" /> '''Pyochelin outer membr...) |
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| - | [[Image:1xkw.gif|left|200px]]<br /><applet load="1xkw" size=" | + | [[Image:1xkw.gif|left|200px]]<br /><applet load="1xkw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xkw, resolution 2.0Å" /> | caption="1xkw, resolution 2.0Å" /> | ||
'''Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa'''<br /> | '''Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pyochelin is a siderophore and virulence factor common to Burkholderia | + | Pyochelin is a siderophore and virulence factor common to Burkholderia cepacia and several Pseudomonas strains. We describe at 2.0 A resolution the crystal structure of the pyochelin outer membrane receptor FptA bound to the iron-pyochelin isolated from Pseudomonas aeruginosa. One pyochelin molecule bound to iron is found in the protein structure, providing the first three-dimensional structure at the atomic level of this siderophore. The pyochelin molecule provides a tetra-dentate coordination of iron, while the remaining bi-dentate coordination is ensured by another molecule not specifically recognized by the protein. The overall structure of the pyochelin receptor is typical of the TonB-dependent transporter superfamily, which uses the proton motive force from the cytoplasmic membrane through the TonB-ExbB-ExbD energy transducing complex to transport ferric ions across the bacterial outer membrane: a transmembrane 22 beta-stranded barrel occluded by a N-terminal domain that contains a mixed four-stranded beta-sheet. The N-terminal TonB box is disordered in two crystal forms, and loop L8 is found to point towards the iron-pyochelin complex, suggesting that the receptor is in a transport-competent conformation. |
==About this Structure== | ==About this Structure== | ||
| - | 1XKW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with SO4, LDA, 188 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1XKW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=LDA:'>LDA</scene>, <scene name='pdbligand=188:'>188</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XKW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tonb dependent receptor]] | [[Category: tonb dependent receptor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:55:53 2008'' |
Revision as of 13:55, 21 February 2008
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Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa
Overview
Pyochelin is a siderophore and virulence factor common to Burkholderia cepacia and several Pseudomonas strains. We describe at 2.0 A resolution the crystal structure of the pyochelin outer membrane receptor FptA bound to the iron-pyochelin isolated from Pseudomonas aeruginosa. One pyochelin molecule bound to iron is found in the protein structure, providing the first three-dimensional structure at the atomic level of this siderophore. The pyochelin molecule provides a tetra-dentate coordination of iron, while the remaining bi-dentate coordination is ensured by another molecule not specifically recognized by the protein. The overall structure of the pyochelin receptor is typical of the TonB-dependent transporter superfamily, which uses the proton motive force from the cytoplasmic membrane through the TonB-ExbB-ExbD energy transducing complex to transport ferric ions across the bacterial outer membrane: a transmembrane 22 beta-stranded barrel occluded by a N-terminal domain that contains a mixed four-stranded beta-sheet. The N-terminal TonB box is disordered in two crystal forms, and loop L8 is found to point towards the iron-pyochelin complex, suggesting that the receptor is in a transport-competent conformation.
About this Structure
1XKW is a Single protein structure of sequence from Pseudomonas aeruginosa with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure at high resolution of ferric-pyochelin and its membrane receptor FptA from Pseudomonas aeruginosa., Cobessi D, Celia H, Pattus F, J Mol Biol. 2005 Sep 30;352(4):893-904. PMID:16139844
Page seeded by OCA on Thu Feb 21 15:55:53 2008
Categories: Pseudomonas aeruginosa | Single protein | Celia, H. | Cobessi, D. | Pattus, F. | 188 | EDO | LDA | SO4 | Tonb dependent receptor
