1xmo

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(New page: 200px<br /><applet load="1xmo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xmo, resolution 3.25&Aring;" /> '''Crystal Structure of...)
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[[Image:1xmo.gif|left|200px]]<br /><applet load="1xmo" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1xmo.gif|left|200px]]<br /><applet load="1xmo" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1xmo, resolution 3.25&Aring;" />
caption="1xmo, resolution 3.25&Aring;" />
'''Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center'''<br />
'''Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center'''<br />
==Overview==
==Overview==
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The natural modification of specific nucleosides in many tRNAs is, essential during decoding of mRNA by the ribosome. For example, tRNA(Lys)(UUU) requires the modification N6-threonylcarbamoyladenosine at, position 37 (t(6)A37), adjacent and 3' to the anticodon, to bind AAA in, the A site of the ribosomal 30S subunit. Moreover, it can only bind both, AAA and AAG lysine codons when doubly modified with t(6)A37 and either, 5-methylaminomethyluridine or 2-thiouridine at the wobble position, (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA, anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons, in the A site. These structures allow the rationalization of how, modifications in the anticodon loop enable decoding of both lysine codons, AAA and AAG.
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The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(Lys)(UUU) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3' to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.
==About this Structure==
==About this Structure==
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1XMO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with PAR, MG and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XMO OCA].
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1XMO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=PAR:'>PAR</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMO OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Agris, P.F.]]
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[[Category: Agris, P F.]]
[[Category: Malkiewicz, A.]]
[[Category: Malkiewicz, A.]]
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[[Category: Murphy, F.V.]]
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[[Category: Murphy, F V.]]
[[Category: Ramakrishnan, V.]]
[[Category: Ramakrishnan, V.]]
[[Category: MG]]
[[Category: MG]]
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[[Category: trna modification]]
[[Category: trna modification]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:10:56 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:56:27 2008''

Revision as of 13:56, 21 February 2008

Image:1xmo.gif

1xmo, resolution 3.25Å

Drag the structure with the mouse to rotate

Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center

Overview

The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(Lys)(UUU) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3' to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.

About this Structure

1XMO is a Protein complex structure of sequences from Thermus thermophilus with , and as ligands. Full crystallographic information is available from OCA.

Reference

The role of modifications in codon discrimination by tRNA(Lys)UUU., Murphy FV 4th, Ramakrishnan V, Malkiewicz A, Agris PF, Nat Struct Mol Biol. 2004 Dec;11(12):1186-91. Epub 2004 Nov 21. PMID:15558052

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