1xs0
From Proteopedia
(New page: 200px<br /><applet load="1xs0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xs0, resolution 1.58Å" /> '''Structure of the E. ...) |
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| - | [[Image:1xs0.jpg|left|200px]]<br /><applet load="1xs0" size=" | + | [[Image:1xs0.jpg|left|200px]]<br /><applet load="1xs0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xs0, resolution 1.58Å" /> | caption="1xs0, resolution 1.58Å" /> | ||
'''Structure of the E. coli Ivy protein'''<br /> | '''Structure of the E. coli Ivy protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The complete nucleotide sequences of over 37 microbial and three eukaryote | + | The complete nucleotide sequences of over 37 microbial and three eukaryote genomes are already publicly available, and more sequencing is in progress. Despite this accumulation of data, newly sequenced microbial genomes continue to reveal up to 50% of functionally uncharacterized "anonymous" genes. A majority of these anonymous proteins have homologues in other organisms, whereas the rest exhibit no clear similarity to any other sequence in the data bases. This set of unique, apparently species-specific, sequences are referred to as ORFans. The biochemical and structural analysis of ORFan gene products is of both evolutionary and functional interest. Here we report the cloning and expression of Escherichia coli ORFan ykfE gene and the functional characterization of the encoded protein. Under physiological conditions, the protein is a homodimer with a strong affinity for C-type lysozyme, as revealed by co-purification and co-crystallization. Activity measurements and fluorescence studies demonstrated that the YkfE gene product is a potent C-type lysozyme inhibitor (K(i) approximately 1 nm). To denote this newly assigned function, ykfE has now been registered under the new gene name Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock center. |
==About this Structure== | ==About this Structure== | ||
| - | 1XS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1XS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Abergel, C.]] | [[Category: Abergel, C.]] | ||
[[Category: Byrn, D.]] | [[Category: Byrn, D.]] | ||
| - | [[Category: Claverie, J | + | [[Category: Claverie, J M.]] |
| - | [[Category: Lazzaroni, J | + | [[Category: Lazzaroni, J C.]] |
[[Category: Monchois, V.]] | [[Category: Monchois, V.]] | ||
[[Category: alpha beta fold]] | [[Category: alpha beta fold]] | ||
[[Category: dimer]] | [[Category: dimer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:02 2008'' |
Revision as of 13:58, 21 February 2008
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Structure of the E. coli Ivy protein
Overview
The complete nucleotide sequences of over 37 microbial and three eukaryote genomes are already publicly available, and more sequencing is in progress. Despite this accumulation of data, newly sequenced microbial genomes continue to reveal up to 50% of functionally uncharacterized "anonymous" genes. A majority of these anonymous proteins have homologues in other organisms, whereas the rest exhibit no clear similarity to any other sequence in the data bases. This set of unique, apparently species-specific, sequences are referred to as ORFans. The biochemical and structural analysis of ORFan gene products is of both evolutionary and functional interest. Here we report the cloning and expression of Escherichia coli ORFan ykfE gene and the functional characterization of the encoded protein. Under physiological conditions, the protein is a homodimer with a strong affinity for C-type lysozyme, as revealed by co-purification and co-crystallization. Activity measurements and fluorescence studies demonstrated that the YkfE gene product is a potent C-type lysozyme inhibitor (K(i) approximately 1 nm). To denote this newly assigned function, ykfE has now been registered under the new gene name Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock center.
About this Structure
1XS0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme., Monchois V, Abergel C, Sturgis J, Jeudy S, Claverie JM, J Biol Chem. 2001 May 25;276(21):18437-41. Epub 2001 Feb 23. PMID:11278658
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