1xsk
From Proteopedia
(New page: 200px<br /><applet load="1xsk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xsk, resolution 2.20Å" /> '''Structure of a Famil...) |
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| - | [[Image:1xsk.gif|left|200px]]<br /><applet load="1xsk" size=" | + | [[Image:1xsk.gif|left|200px]]<br /><applet load="1xsk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xsk, resolution 2.20Å" /> | caption="1xsk, resolution 2.20Å" /> | ||
'''Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate'''<br /> | '''Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a | + | We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp(416) to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease. |
==About this Structure== | ==About this Structure== | ||
| - | 1XSK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, XYF and MPO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1XSK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=XYF:'>XYF</scene> and <scene name='pdbligand=MPO:'>MPO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kim, Y | + | [[Category: Kim, Y W.]] |
| - | [[Category: Lee, S | + | [[Category: Lee, S S.]] |
| - | [[Category: Lovering, A | + | [[Category: Lovering, A L.]] |
| - | [[Category: Strynadka, N | + | [[Category: Strynadka, N C.]] |
| - | [[Category: Withers, S | + | [[Category: Withers, S G.]] |
[[Category: MPO]] | [[Category: MPO]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: beta(8)alpha(8) barrel and beta sandwich domains]] | [[Category: beta(8)alpha(8) barrel and beta sandwich domains]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:20 2008'' |
Revision as of 13:58, 21 February 2008
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Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate
Overview
We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp(416) to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease.
About this Structure
1XSK is a Single protein structure of sequence from Escherichia coli with , and as ligands. Full crystallographic information is available from OCA.
Reference
Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate., Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC, J Biol Chem. 2005 Jan 21;280(3):2105-15. Epub 2004 Oct 22. PMID:15501829
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