1xt6
From Proteopedia
(New page: 200px<br /><applet load="1xt6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xt6, resolution 1.80Å" /> '''S35C Flavodoxin Muta...) |
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| - | [[Image:1xt6.gif|left|200px]]<br /><applet load="1xt6" size=" | + | [[Image:1xt6.gif|left|200px]]<br /><applet load="1xt6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xt6, resolution 1.80Å" /> | caption="1xt6, resolution 1.80Å" /> | ||
'''S35C Flavodoxin Mutant in the semiquinone state'''<br /> | '''S35C Flavodoxin Mutant in the semiquinone state'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystallographic structure of an engineered flavodoxin mutant from | + | The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1XT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1XT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Marchini, N.]] | [[Category: Marchini, N.]] | ||
[[Category: Meneghetti, F.]] | [[Category: Meneghetti, F.]] | ||
| - | [[Category: Rossi, G | + | [[Category: Rossi, G L.]] |
[[Category: Sassone, C.]] | [[Category: Sassone, C.]] | ||
[[Category: FMN]] | [[Category: FMN]] | ||
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[[Category: s35c]] | [[Category: s35c]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:23 2008'' |
Revision as of 13:58, 21 February 2008
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S35C Flavodoxin Mutant in the semiquinone state
Overview
The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.
About this Structure
1XT6 is a Single protein structure of sequence from Desulfovibrio vulgaris with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state., Artali R, Marchini N, Meneghetti F, Cavazzini D, Cassetta A, Sassone C, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):481-4. Epub 2005, Mar 24. PMID:15805604
Page seeded by OCA on Thu Feb 21 15:58:23 2008
Categories: Desulfovibrio vulgaris | Single protein | Artali, R. | Bombieri, G. | Cassetta, A. | Cavazzini, D. | Gilardi, G. | Marchini, N. | Meneghetti, F. | Rossi, G L. | Sassone, C. | FMN | Flavodoxin | Mutant | Protein | S35c
