1xsn
From Proteopedia
(New page: 200px<br /> <applet load="1xsn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xsn, resolution 1.95Å" /> '''Crystal Structure o...) |
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| - | [[Image:1xsn.gif|left|200px]]<br /> | + | [[Image:1xsn.gif|left|200px]]<br /><applet load="1xsn" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1xsn" size=" | + | |
caption="1xsn, resolution 1.95Å" /> | caption="1xsn, resolution 1.95Å" /> | ||
'''Crystal Structure of human DNA polymerase lambda in complex with a one nucleotide DNA gap and ddTTP'''<br /> | '''Crystal Structure of human DNA polymerase lambda in complex with a one nucleotide DNA gap and ddTTP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pol lambda is a family X member believed to fill short gaps during DNA | + | Pol lambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol lambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol lambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction. |
==About this Structure== | ==About this Structure== | ||
| - | 1XSN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, NA, D3T and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1XSN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=D3T:'>D3T</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bebenek, K.]] | [[Category: Bebenek, K.]] | ||
[[Category: Garcia-Diaz, M.]] | [[Category: Garcia-Diaz, M.]] | ||
| - | [[Category: Krahn, J | + | [[Category: Krahn, J M.]] |
| - | [[Category: Kunkel, T | + | [[Category: Kunkel, T A.]] |
| - | [[Category: Pedersen, L | + | [[Category: Pedersen, L C.]] |
[[Category: D3T]] | [[Category: D3T]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:14 2008'' |
Revision as of 13:58, 21 February 2008
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Crystal Structure of human DNA polymerase lambda in complex with a one nucleotide DNA gap and ddTTP
Overview
Pol lambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol lambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol lambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.
About this Structure
1XSN is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
A closed conformation for the Pol lambda catalytic cycle., Garcia-Diaz M, Bebenek K, Krahn JM, Kunkel TA, Pedersen LC, Nat Struct Mol Biol. 2005 Jan;12(1):97-8. Epub 2004 Dec 19. PMID:15608652
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