1xta
From Proteopedia
(New page: 200px<br /><applet load="1xta" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xta, resolution 1.58Å" /> '''Crystal Structure of...) |
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| - | [[Image:1xta.gif|left|200px]]<br /><applet load="1xta" size=" | + | [[Image:1xta.gif|left|200px]]<br /><applet load="1xta" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xta, resolution 1.58Å" /> | caption="1xta, resolution 1.58Å" /> | ||
'''Crystal Structure of Natrin, a snake venom CRISP from Taiwan cobra (Naja atra)'''<br /> | '''Crystal Structure of Natrin, a snake venom CRISP from Taiwan cobra (Naja atra)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cysteine-rich secretory proteins (CRISPs) play an important role in the | + | Cysteine-rich secretory proteins (CRISPs) play an important role in the innate immune system and are transcriptionally regulated by androgens in several tissues. The proteins are mostly found in the epididymis and granules of mammals, whilst a number of snake venoms also contain CRISP-family proteins. The natrin protein from the venom of Naja atra (Taiwan cobra), which belongs to a family of CRISPs and has a cysteine-rich C-terminal amino-acid sequence, has been purified using a three-stage chromatography procedure and crystals suitable for X-ray analysis have been obtained using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.58 A resolution using synchrotron radiation; the crystals belong to space group C222(1), with unit-cell parameters a = 59.172, b = 65.038, c = 243.156 A. There are two protein molecules in the asymmetric unit and the Matthews coefficient is estimated to be 2.35 A3 Da(-1), corresponding to a solvent content of 47.60%. |
==About this Structure== | ==About this Structure== | ||
| - | 1XTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http:// | + | 1XTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Naja atra]] | [[Category: Naja atra]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chen, C | + | [[Category: Chen, C J.]] |
| - | [[Category: Goh, K | + | [[Category: Goh, K X.]] |
| - | [[Category: Huang, W | + | [[Category: Huang, W N.]] |
| - | [[Category: Lee, S | + | [[Category: Lee, S C.]] |
| - | [[Category: Wang, Y | + | [[Category: Wang, Y L.]] |
| - | [[Category: Wu, W | + | [[Category: Wu, W G.]] |
[[Category: cobra]] | [[Category: cobra]] | ||
[[Category: crisp]] | [[Category: crisp]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:25 2008'' |
Revision as of 13:58, 21 February 2008
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Crystal Structure of Natrin, a snake venom CRISP from Taiwan cobra (Naja atra)
Overview
Cysteine-rich secretory proteins (CRISPs) play an important role in the innate immune system and are transcriptionally regulated by androgens in several tissues. The proteins are mostly found in the epididymis and granules of mammals, whilst a number of snake venoms also contain CRISP-family proteins. The natrin protein from the venom of Naja atra (Taiwan cobra), which belongs to a family of CRISPs and has a cysteine-rich C-terminal amino-acid sequence, has been purified using a three-stage chromatography procedure and crystals suitable for X-ray analysis have been obtained using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.58 A resolution using synchrotron radiation; the crystals belong to space group C222(1), with unit-cell parameters a = 59.172, b = 65.038, c = 243.156 A. There are two protein molecules in the asymmetric unit and the Matthews coefficient is estimated to be 2.35 A3 Da(-1), corresponding to a solvent content of 47.60%.
About this Structure
1XTA is a Single protein structure of sequence from Naja atra. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary X-ray crystallographic analysis of a cysteine-rich secretory protein (CRISP) from Naja atra venom., Wang YL, Goh KX, Wu WG, Chen CJ, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1912-5. Epub 2004, Sep 23. PMID:15388950
Page seeded by OCA on Thu Feb 21 15:58:25 2008
Categories: Naja atra | Single protein | Chen, C J. | Goh, K X. | Huang, W N. | Lee, S C. | Wang, Y L. | Wu, W G. | Cobra | Crisp | Ion channel blocking | Natrin | Serine protease
