1xt7
From Proteopedia
(New page: 200px<br /><applet load="1xt7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xt7" /> '''Daptomycin NMR Structure'''<br /> ==Overvie...) |
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| - | [[Image:1xt7.gif|left|200px]]<br /><applet load="1xt7" size=" | + | [[Image:1xt7.gif|left|200px]]<br /><applet load="1xt7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xt7" /> | caption="1xt7" /> | ||
'''Daptomycin NMR Structure'''<br /> | '''Daptomycin NMR Structure'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Daptomycin is an acidic lipopeptide antibiotic, whose three-dimensional | + | Daptomycin is an acidic lipopeptide antibiotic, whose three-dimensional structure and mechanism of action is currently unknown. Recently daptomycin, trade name Cubicin, was approved as a drug for the treatment of skin-related infections (M. Larkin Lancet, 2003, 3, 677) and became the first antibiotic of its class to be used in the clinic (A. Raja et al., Nature Rev. Drug Discov., 2003, 2, 943-944). We have carried out a systematic high field NMR study of daptomycin and its binding to calcium ions which is essential for antibiotic activity. In this first report, we demonstrate the sequence-specific resonance assignment of daptomycin under resolved NMR measurement conditions. In addition to this, we have determined the 3D structure of apo-daptomycin and demonstrated a 1 : 1 stoichiometry on the binding to calcium ions. We have also demonstrated that the binding of calcium ions does not result in major conformational changes, but does induce aggregation. This may be an important factor in the mode of action of daptomycin. |
==About this Structure== | ==About this Structure== | ||
| - | 1XT7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1XT7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT7 OCA]. |
==Reference== | ==Reference== | ||
NMR structure determination and calcium binding effects of lipopeptide antibiotic daptomycin., Ball LJ, Goult CM, Donarski JA, Micklefield J, Ramesh V, Org Biomol Chem. 2004 Jul 7;2(13):1872-8. Epub 2004 Jun 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15227539 15227539] | NMR structure determination and calcium binding effects of lipopeptide antibiotic daptomycin., Ball LJ, Goult CM, Donarski JA, Micklefield J, Ramesh V, Org Biomol Chem. 2004 Jul 7;2(13):1872-8. Epub 2004 Jun 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15227539 15227539] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Ball, L | + | [[Category: Ball, L J.]] |
| - | [[Category: Donarski, J | + | [[Category: Donarski, J A.]] |
| - | [[Category: Goult, C | + | [[Category: Goult, C M.]] |
[[Category: Micklefield, J.]] | [[Category: Micklefield, J.]] | ||
[[Category: Ramesh, V.]] | [[Category: Ramesh, V.]] | ||
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[[Category: lipopeptide]] | [[Category: lipopeptide]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:23 2008'' |
Revision as of 13:58, 21 February 2008
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Daptomycin NMR Structure
Overview
Daptomycin is an acidic lipopeptide antibiotic, whose three-dimensional structure and mechanism of action is currently unknown. Recently daptomycin, trade name Cubicin, was approved as a drug for the treatment of skin-related infections (M. Larkin Lancet, 2003, 3, 677) and became the first antibiotic of its class to be used in the clinic (A. Raja et al., Nature Rev. Drug Discov., 2003, 2, 943-944). We have carried out a systematic high field NMR study of daptomycin and its binding to calcium ions which is essential for antibiotic activity. In this first report, we demonstrate the sequence-specific resonance assignment of daptomycin under resolved NMR measurement conditions. In addition to this, we have determined the 3D structure of apo-daptomycin and demonstrated a 1 : 1 stoichiometry on the binding to calcium ions. We have also demonstrated that the binding of calcium ions does not result in major conformational changes, but does induce aggregation. This may be an important factor in the mode of action of daptomycin.
About this Structure
1XT7 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR structure determination and calcium binding effects of lipopeptide antibiotic daptomycin., Ball LJ, Goult CM, Donarski JA, Micklefield J, Ramesh V, Org Biomol Chem. 2004 Jul 7;2(13):1872-8. Epub 2004 Jun 15. PMID:15227539
Page seeded by OCA on Thu Feb 21 15:58:23 2008
