1xtj
From Proteopedia
(New page: 200px<br /> <applet load="1xtj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xtj, resolution 2.70Å" /> '''structure of human ...) |
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| - | [[Image:1xtj.gif|left|200px]]<br /> | + | [[Image:1xtj.gif|left|200px]]<br /><applet load="1xtj" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1xtj" size=" | + | |
caption="1xtj, resolution 2.70Å" /> | caption="1xtj, resolution 2.70Å" /> | ||
'''structure of human UAP56 in complex with ADP'''<br /> | '''structure of human UAP56 in complex with ADP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pre-mRNA splicing requires the function of a number of RNA-dependent | + | Pre-mRNA splicing requires the function of a number of RNA-dependent ATPases/helicases, yet no three-dimensional structure of any spliceosomal ATPases/helicases is known. The highly conserved DECD-box protein UAP56/Sub2 is an essential splicing factor that is also important for mRNA export. The expected ATPase/helicase activity appears to be essential for the UAP56/Sub2 functions. Here, we show that purified human UAP56 is an active RNA-dependent ATPase, and we also report the crystal structures of UAP56 alone and in complex with ADP, as well as a DECD to DEAD mutant. The structures reveal a unique spatial arrangement of the two conserved helicase domains, and ADP-binding induces significant conformational changes of key residues in the ATP-binding pocket. Our structural analyses suggest a specific protein-RNA displacement model of UAP56/Sub2. The detailed structural information provides important mechanistic insights into the splicing function of UAP56/Sub2. The structures also will be useful for the analysis of other spliceosomal DExD-box ATPases/helicases. |
==About this Structure== | ==About this Structure== | ||
| - | 1XTJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, ADP and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1XTJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cordin, O.]] | [[Category: Cordin, O.]] | ||
[[Category: Linder, P.]] | [[Category: Linder, P.]] | ||
| - | [[Category: Minder, C | + | [[Category: Minder, C M.]] |
[[Category: Shi, H.]] | [[Category: Shi, H.]] | ||
| - | [[Category: Xu, R | + | [[Category: Xu, R M.]] |
[[Category: ACY]] | [[Category: ACY]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
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[[Category: alpha-beta fold]] | [[Category: alpha-beta fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:34 2008'' |
Revision as of 13:58, 21 February 2008
|
structure of human UAP56 in complex with ADP
Overview
Pre-mRNA splicing requires the function of a number of RNA-dependent ATPases/helicases, yet no three-dimensional structure of any spliceosomal ATPases/helicases is known. The highly conserved DECD-box protein UAP56/Sub2 is an essential splicing factor that is also important for mRNA export. The expected ATPase/helicase activity appears to be essential for the UAP56/Sub2 functions. Here, we show that purified human UAP56 is an active RNA-dependent ATPase, and we also report the crystal structures of UAP56 alone and in complex with ADP, as well as a DECD to DEAD mutant. The structures reveal a unique spatial arrangement of the two conserved helicase domains, and ADP-binding induces significant conformational changes of key residues in the ATP-binding pocket. Our structural analyses suggest a specific protein-RNA displacement model of UAP56/Sub2. The detailed structural information provides important mechanistic insights into the splicing function of UAP56/Sub2. The structures also will be useful for the analysis of other spliceosomal DExD-box ATPases/helicases.
About this Structure
1XTJ is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the human ATP-dependent splicing and export factor UAP56., Shi H, Cordin O, Minder CM, Linder P, Xu RM, Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17628-33. Epub 2004 Dec 7. PMID:15585580
Page seeded by OCA on Thu Feb 21 15:58:34 2008
Categories: Homo sapiens | Single protein | Cordin, O. | Linder, P. | Minder, C M. | Shi, H. | Xu, R M. | ACY | ADP | MG | Alpha-beta fold
