1y0q
From Proteopedia
(New page: 200px<br /><applet load="1y0q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y0q, resolution 3.60Å" /> '''Crystal structure of...) |
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| - | [[Image:1y0q.gif|left|200px]]<br /><applet load="1y0q" size=" | + | [[Image:1y0q.gif|left|200px]]<br /><applet load="1y0q" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1y0q, resolution 3.60Å" /> | caption="1y0q, resolution 3.60Å" /> | ||
'''Crystal structure of an active group I ribozyme-product complex'''<br /> | '''Crystal structure of an active group I ribozyme-product complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Group I introns are catalytic RNAs capable of orchestrating two sequential | + | Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis. |
==About this Structure== | ==About this Structure== | ||
| - | 1Y0Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort] with SPK, MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1Y0Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort] with <scene name='pdbligand=SPK:'>SPK</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Staphylococcus phage twort]] | [[Category: Staphylococcus phage twort]] | ||
[[Category: Chase, E.]] | [[Category: Chase, E.]] | ||
| - | [[Category: Golden, B | + | [[Category: Golden, B L.]] |
[[Category: Kim, H.]] | [[Category: Kim, H.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: rna-splicing]] | [[Category: rna-splicing]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:00:39 2008'' |
Revision as of 14:00, 21 February 2008
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Crystal structure of an active group I ribozyme-product complex
Overview
Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis.
About this Structure
1Y0Q is a Protein complex structure of sequences from Staphylococcus phage twort with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:15580277
Page seeded by OCA on Thu Feb 21 16:00:39 2008
Categories: Protein complex | Staphylococcus phage twort | Chase, E. | Golden, B L. | Kim, H. | MG | SO4 | SPK | Guanosine-binding | Ribozyme | Rna-folding | Rna-splicing
