1y8x
From Proteopedia
(New page: 200px<br /> <applet load="1y8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y8x, resolution 2.4Å" /> '''Structural basis for...) |
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| - | [[Image:1y8x.gif|left|200px]]<br /> | + | [[Image:1y8x.gif|left|200px]]<br /><applet load="1y8x" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1y8x, resolution 2.4Å" /> | caption="1y8x, resolution 2.4Å" /> | ||
'''Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1'''<br /> | '''Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | E2 conjugating enzymes play a central role in ubiquitin and ubiquitin-like | + | E2 conjugating enzymes play a central role in ubiquitin and ubiquitin-like protein (ublp) transfer cascades: the E2 accepts the ublp from the E1 enzyme and then the E2 often interacts with an E3 enzyme to promote ublp transfer to the target. We report here the crystal structure of a complex between the C-terminal domain from NEDD8's heterodimeric E1 (APPBP1-UBA3) and the catalytic core domain of NEDD8's E2 (Ubc12). The structure and associated mutational analyses reveal molecular details of Ubc12 recruitment by NEDD8's E1. Interestingly, the E1's Ubc12 binding domain resembles ubiquitin and recruits Ubc12 in a manner mimicking ubiquitin's interactions with ubiquitin binding domains. Structural comparison with E2-E3 complexes indicates that the E1 and E3 binding sites on Ubc12 may overlap and raises the possibility that crosstalk between E1 and E3 interacting with an E2 could influence the specificity and processivity of ublp transfer. |
==About this Structure== | ==About this Structure== | ||
| - | 1Y8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http:// | + | 1Y8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y8X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Ubiquitin--protein ligase]] | [[Category: Ubiquitin--protein ligase]] | ||
| - | [[Category: Holton, J | + | [[Category: Holton, J M.]] |
| - | [[Category: Huang, D | + | [[Category: Huang, D T.]] |
[[Category: Paydar, A.]] | [[Category: Paydar, A.]] | ||
| - | [[Category: Schulman, B | + | [[Category: Schulman, B A.]] |
| - | [[Category: Waddell, M | + | [[Category: Waddell, M B.]] |
[[Category: Zhuang, M.]] | [[Category: Zhuang, M.]] | ||
[[Category: ligase]] | [[Category: ligase]] | ||
[[Category: ubiquitin-conjugating enzyme e2 m ]] | [[Category: ubiquitin-conjugating enzyme e2 m ]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:03:01 2008'' |
Revision as of 14:03, 21 February 2008
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Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1
Overview
E2 conjugating enzymes play a central role in ubiquitin and ubiquitin-like protein (ublp) transfer cascades: the E2 accepts the ublp from the E1 enzyme and then the E2 often interacts with an E3 enzyme to promote ublp transfer to the target. We report here the crystal structure of a complex between the C-terminal domain from NEDD8's heterodimeric E1 (APPBP1-UBA3) and the catalytic core domain of NEDD8's E2 (Ubc12). The structure and associated mutational analyses reveal molecular details of Ubc12 recruitment by NEDD8's E1. Interestingly, the E1's Ubc12 binding domain resembles ubiquitin and recruits Ubc12 in a manner mimicking ubiquitin's interactions with ubiquitin binding domains. Structural comparison with E2-E3 complexes indicates that the E1 and E3 binding sites on Ubc12 may overlap and raises the possibility that crosstalk between E1 and E3 interacting with an E2 could influence the specificity and processivity of ublp transfer.
About this Structure
1Y8X is a Protein complex structure of sequences from Homo sapiens. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Full crystallographic information is available from OCA.
Reference
Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1., Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA, Mol Cell. 2005 Feb 4;17(3):341-50. PMID:15694336
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