1ygm
From Proteopedia
(New page: 200px<br /><applet load="1ygm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ygm" /> '''NMR structure of Mistic'''<br /> ==Overview...) |
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| - | [[Image:1ygm.gif|left|200px]]<br /><applet load="1ygm" size=" | + | [[Image:1ygm.gif|left|200px]]<br /><applet load="1ygm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ygm" /> | caption="1ygm" /> | ||
'''NMR structure of Mistic'''<br /> | '''NMR structure of Mistic'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental | + | Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression. |
==About this Structure== | ==About this Structure== | ||
| - | 1YGM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http:// | + | 1YGM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Greenwald, J.]] | [[Category: Greenwald, J.]] | ||
[[Category: Riek, R.]] | [[Category: Riek, R.]] | ||
| - | [[Category: Roosild, T | + | [[Category: Roosild, T P.]] |
[[Category: Vega, M.]] | [[Category: Vega, M.]] | ||
[[Category: alpha-helical bundle]] | [[Category: alpha-helical bundle]] | ||
[[Category: integral membrane protein]] | [[Category: integral membrane protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:05:13 2008'' |
Revision as of 14:05, 21 February 2008
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NMR structure of Mistic
Overview
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
About this Structure
1YGM is a Single protein structure of sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA.
Reference
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457
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