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1ym0
From Proteopedia
(New page: 200px<br /><applet load="1ym0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ym0, resolution 2.06Å" /> '''Crystal Structure of...) |
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| - | [[Image:1ym0.gif|left|200px]]<br /><applet load="1ym0" size=" | + | [[Image:1ym0.gif|left|200px]]<br /><applet load="1ym0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ym0, resolution 2.06Å" /> | caption="1ym0, resolution 2.06Å" /> | ||
'''Crystal Structure of Earthworm Fibrinolytic Enzyme Component B: a Novel, Glycosylated Two-chained Trypsin'''<br /> | '''Crystal Structure of Earthworm Fibrinolytic Enzyme Component B: a Novel, Glycosylated Two-chained Trypsin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The earthworm fibrinolytic enzyme (EFE), belonging to a group of serine | + | The earthworm fibrinolytic enzyme (EFE), belonging to a group of serine proteases with strong fibrinolytic activity, has been used in a mixture as an oral drug for prevention and treatment of thrombosis in East Asia. The EFE component b (EFE-b) is one of seven EFE components from Eisenia fetida, and among them it has nearly the highest fibrinolytic activity. Here, we report its crystal structure at a resolution of 2.06A. The structural analysis shows that EFE-b should be classified as a trypsin from earthworm. However, it is distinct from other trypsins. It is a two-chained protease with an N-terminal, pyroglutamated light chain and an N-glycosylated heavy chain. Furthermore, the heavy chain contains a novel structural motif, an eight-membered ring resulting from a disulfide bridge between two neighboring cysteine residues, and a cis peptide bond exists between these two cysteine residues. The crystal structure of EFE-b provides the structural basis for its high level of stability and reveals its complicated post-translational modifications in earthworm. This structure is the first reported for a glycosylated two-chained trypsin, which may provide useful clues to explain the origin and evolution of the chymotrypsin family. |
==About this Structure== | ==About this Structure== | ||
| - | 1YM0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Eisenia_fetida Eisenia fetida] with MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1YM0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Eisenia_fetida Eisenia fetida] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YM0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Eisenia fetida]] | [[Category: Eisenia fetida]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: An, X | + | [[Category: An, X M.]] |
| - | [[Category: Chang, W | + | [[Category: Chang, W R.]] |
| - | [[Category: Gui, L | + | [[Category: Gui, L L.]] |
[[Category: Li, M.]] | [[Category: Li, M.]] | ||
[[Category: Wang, C.]] | [[Category: Wang, C.]] | ||
[[Category: Wang, F.]] | [[Category: Wang, F.]] | ||
| - | [[Category: Zhang, J | + | [[Category: Zhang, J P.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: two chains]] | [[Category: two chains]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:42 2008'' |
Revision as of 14:06, 21 February 2008
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Crystal Structure of Earthworm Fibrinolytic Enzyme Component B: a Novel, Glycosylated Two-chained Trypsin
Overview
The earthworm fibrinolytic enzyme (EFE), belonging to a group of serine proteases with strong fibrinolytic activity, has been used in a mixture as an oral drug for prevention and treatment of thrombosis in East Asia. The EFE component b (EFE-b) is one of seven EFE components from Eisenia fetida, and among them it has nearly the highest fibrinolytic activity. Here, we report its crystal structure at a resolution of 2.06A. The structural analysis shows that EFE-b should be classified as a trypsin from earthworm. However, it is distinct from other trypsins. It is a two-chained protease with an N-terminal, pyroglutamated light chain and an N-glycosylated heavy chain. Furthermore, the heavy chain contains a novel structural motif, an eight-membered ring resulting from a disulfide bridge between two neighboring cysteine residues, and a cis peptide bond exists between these two cysteine residues. The crystal structure of EFE-b provides the structural basis for its high level of stability and reveals its complicated post-translational modifications in earthworm. This structure is the first reported for a glycosylated two-chained trypsin, which may provide useful clues to explain the origin and evolution of the chymotrypsin family.
About this Structure
1YM0 is a Protein complex structure of sequences from Eisenia fetida with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of earthworm fibrinolytic enzyme component B: a novel, glycosylated two-chained trypsin., Wang F, Wang C, Li M, Zhang JP, Gui LL, An XM, Chang WR, J Mol Biol. 2005 May 6;348(3):671-85. PMID:15826663
Page seeded by OCA on Thu Feb 21 16:06:42 2008
Categories: Eisenia fetida | Protein complex | An, X M. | Chang, W R. | Gui, L L. | Li, M. | Wang, C. | Wang, F. | Zhang, J P. | MG | SO4 | Cis peptide bond | Eight-membered ring | Glycosylation | Pyroglutamation | Two chains
