1ymp
From Proteopedia
(New page: 200px<br /><applet load="1ymp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ymp, resolution 2.200Å" /> '''The Crystal Structu...) |
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| - | [[Image:1ymp.gif|left|200px]]<br /><applet load="1ymp" size=" | + | [[Image:1ymp.gif|left|200px]]<br /><applet load="1ymp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ymp, resolution 2.200Å" /> | caption="1ymp, resolution 2.200Å" /> | ||
'''The Crystal Structure of a Partial Mouse Notch-1 Ankyrin Domain: Repeats 4 Through 7 Preserve an Ankyrin Fold'''<br /> | '''The Crystal Structure of a Partial Mouse Notch-1 Ankyrin Domain: Repeats 4 Through 7 Preserve an Ankyrin Fold'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Folding and stability of proteins containing ankyrin repeats (ARs) is of | + | Folding and stability of proteins containing ankyrin repeats (ARs) is of great interest because they mediate numerous protein-protein interactions involved in a wide range of regulatory cellular processes. Notch, an ankyrin domain containing protein, signals by converting a transcriptional repression complex into an activation complex. The Notch ANK domain is essential for Notch function and contains seven ARs. Here, we present the 2.2 A crystal structure of ARs 4-7 from mouse Notch 1 (m1ANK). These C-terminal repeats were resistant to degradation during crystallization, and their secondary and tertiary structures are maintained in the absence of repeats 1-3. The crystallized fragment adopts a typical ankyrin fold including the poorly conserved seventh AR, as seen in the Drosophila Notch ANK domain (dANK). The structural preservation and stability of the C-terminal repeats shed a new light onto the mechanism of hetero-oligomeric assembly during Notch-mediated transcriptional activation. |
==About this Structure== | ==About this Structure== | ||
| - | 1YMP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1YMP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kopan, R.]] | [[Category: Kopan, R.]] | ||
[[Category: Korolev, S.]] | [[Category: Korolev, S.]] | ||
| - | [[Category: Lubman, O | + | [[Category: Lubman, O Y.]] |
[[Category: Waksman, G.]] | [[Category: Waksman, G.]] | ||
[[Category: ankyrin repeats]] | [[Category: ankyrin repeats]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:53 2008'' |
Revision as of 14:06, 21 February 2008
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The Crystal Structure of a Partial Mouse Notch-1 Ankyrin Domain: Repeats 4 Through 7 Preserve an Ankyrin Fold
Overview
Folding and stability of proteins containing ankyrin repeats (ARs) is of great interest because they mediate numerous protein-protein interactions involved in a wide range of regulatory cellular processes. Notch, an ankyrin domain containing protein, signals by converting a transcriptional repression complex into an activation complex. The Notch ANK domain is essential for Notch function and contains seven ARs. Here, we present the 2.2 A crystal structure of ARs 4-7 from mouse Notch 1 (m1ANK). These C-terminal repeats were resistant to degradation during crystallization, and their secondary and tertiary structures are maintained in the absence of repeats 1-3. The crystallized fragment adopts a typical ankyrin fold including the poorly conserved seventh AR, as seen in the Drosophila Notch ANK domain (dANK). The structural preservation and stability of the C-terminal repeats shed a new light onto the mechanism of hetero-oligomeric assembly during Notch-mediated transcriptional activation.
About this Structure
1YMP is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The crystal structure of a partial mouse Notch-1 ankyrin domain: repeats 4 through 7 preserve an ankyrin fold., Lubman OY, Kopan R, Waksman G, Korolev S, Protein Sci. 2005 May;14(5):1274-81. Epub 2005 Mar 31. PMID:15802643
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