1yod
From Proteopedia
(New page: 200px<br /><applet load="1yod" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yod, resolution 1.80Å" /> '''Crystal structure of...) |
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| - | [[Image:1yod.gif|left|200px]]<br /><applet load="1yod" size=" | + | [[Image:1yod.gif|left|200px]]<br /><applet load="1yod" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yod, resolution 1.80Å" /> | caption="1yod, resolution 1.80Å" /> | ||
'''Crystal structure of a water soluble analog of phospholamban'''<br /> | '''Crystal structure of a water soluble analog of phospholamban'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Phospholamban (PLB) is a pentameric transmembrane protein that regulates | + | Phospholamban (PLB) is a pentameric transmembrane protein that regulates the Ca(2+)-dependent ATPase SERCA2a in sarcoplasmic reticulum membranes. We previously described the computational design of a water-soluble variant of phospholamban, WSPLB, which reproduced many of the structural and functional properties of the native membrane-soluble protein. While the full-length WSPLB forms a pentamer in solution, a truncated variant forms very stable tetramers. To obtain insight into the tetramer-pentamer cytoplasmic switch, we solved the crystal structure of the truncated construct, WSPLB 21-52. This peptide has a heptad sequence repeat with Leu residues at a- and Ile at d-positions from residues 31-52. The crystal structure revealed that WSPLB 21-52 adopted an antiparallel tetrameric coiled coil. This topology contrasts with the parallel topology of an analogue of the coiled-coil of GCN4 with the same Leu(a) Ile(d) repeat. Analysis of these structures revealed how the nature of the partially exposed residues at e- and g-positions influence the topology formed by the bundle. We also constructed a model for the pentameric form of PLB using the coiled-coil parameters derived from a single monomer in the tetrameric structure. This model suggests that both buried and interfacial hydrogen bonds are important for stabilizing the parallel pentamer. |
==About this Structure== | ==About this Structure== | ||
| - | 1YOD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1YOD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOD OCA]. |
==Reference== | ==Reference== | ||
X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15826670 15826670] | X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15826670 15826670] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: DeGrado, W | + | [[Category: DeGrado, W F.]] |
[[Category: North, B.]] | [[Category: North, B.]] | ||
| - | [[Category: Slovic, A | + | [[Category: Slovic, A M.]] |
| - | [[Category: Stayrook, S | + | [[Category: Stayrook, S E.]] |
[[Category: protein design]] | [[Category: protein design]] | ||
[[Category: water-soluble]] | [[Category: water-soluble]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:25 2008'' |
Revision as of 14:07, 21 February 2008
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Crystal structure of a water soluble analog of phospholamban
Overview
Phospholamban (PLB) is a pentameric transmembrane protein that regulates the Ca(2+)-dependent ATPase SERCA2a in sarcoplasmic reticulum membranes. We previously described the computational design of a water-soluble variant of phospholamban, WSPLB, which reproduced many of the structural and functional properties of the native membrane-soluble protein. While the full-length WSPLB forms a pentamer in solution, a truncated variant forms very stable tetramers. To obtain insight into the tetramer-pentamer cytoplasmic switch, we solved the crystal structure of the truncated construct, WSPLB 21-52. This peptide has a heptad sequence repeat with Leu residues at a- and Ile at d-positions from residues 31-52. The crystal structure revealed that WSPLB 21-52 adopted an antiparallel tetrameric coiled coil. This topology contrasts with the parallel topology of an analogue of the coiled-coil of GCN4 with the same Leu(a) Ile(d) repeat. Analysis of these structures revealed how the nature of the partially exposed residues at e- and g-positions influence the topology formed by the bundle. We also constructed a model for the pentameric form of PLB using the coiled-coil parameters derived from a single monomer in the tetrameric structure. This model suggests that both buried and interfacial hydrogen bonds are important for stabilizing the parallel pentamer.
About this Structure
1YOD is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:15826670
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