1yoe
From Proteopedia
(New page: 200px<br /><applet load="1yoe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yoe, resolution 1.78Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1yoe.gif|left|200px]]<br /><applet load="1yoe" size=" | + | [[Image:1yoe.gif|left|200px]]<br /><applet load="1yoe" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yoe, resolution 1.78Å" /> | caption="1yoe, resolution 1.78Å" /> | ||
'''Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose'''<br /> | '''Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Enzymes with nucleoside hydrolase (NH) activity are crucial for salvaging | + | Enzymes with nucleoside hydrolase (NH) activity are crucial for salvaging nucleic acid components in purine auxotrophic protozoan parasites, but are also present in prokaryotes and higher eukaryotes. Here we analyze the distribution of genes encoding for putative NH proteins and characterize the yeiK gene product from Escherichia coli as a pyrimidine-specific NH. The crystal structure of YeiK to 1.7 A defines the structural basis for its substrate specificity and identifies residues involved in the catalytic mechanism that differ from both nonspecific and purine-specific NHs. Large differences in the tetrameric quaternary structure compared to nonspecific protozoan NHs are brought forth by minor differences in the interacting surfaces. The first structural and functional characterization of a nonparasitic, pyrimidine nucleoside-specific NH suggests a possible role for these enzymes in the metabolism of tRNA nucleosides. The high catalytic efficiency of YeiK toward 5-fluorouridine could be exploited for suicide gene therapy in cancer treatment. |
==About this Structure== | ==About this Structure== | ||
| - | 1YOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with RIB and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribosylpyrimidine_nucleosidase Ribosylpyrimidine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.8 3.2.2.8] Full crystallographic information is available from [http:// | + | 1YOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=RIB:'>RIB</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribosylpyrimidine_nucleosidase Ribosylpyrimidine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.8 3.2.2.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOE OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 25: | ||
[[Category: ribose]] | [[Category: ribose]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:28 2008'' |
Revision as of 14:07, 21 February 2008
|
Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose
Overview
Enzymes with nucleoside hydrolase (NH) activity are crucial for salvaging nucleic acid components in purine auxotrophic protozoan parasites, but are also present in prokaryotes and higher eukaryotes. Here we analyze the distribution of genes encoding for putative NH proteins and characterize the yeiK gene product from Escherichia coli as a pyrimidine-specific NH. The crystal structure of YeiK to 1.7 A defines the structural basis for its substrate specificity and identifies residues involved in the catalytic mechanism that differ from both nonspecific and purine-specific NHs. Large differences in the tetrameric quaternary structure compared to nonspecific protozoan NHs are brought forth by minor differences in the interacting surfaces. The first structural and functional characterization of a nonparasitic, pyrimidine nucleoside-specific NH suggests a possible role for these enzymes in the metabolism of tRNA nucleosides. The high catalytic efficiency of YeiK toward 5-fluorouridine could be exploited for suicide gene therapy in cancer treatment.
About this Structure
1YOE is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Ribosylpyrimidine nucleosidase, with EC number 3.2.2.8 Full crystallographic information is available from OCA.
Reference
Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy., Giabbai B, Degano M, Structure. 2004 May;12(5):739-49. PMID:15130467
Page seeded by OCA on Thu Feb 21 16:07:28 2008
