1yp0
From Proteopedia
(New page: 200px<br /><applet load="1yp0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yp0, resolution 1.50Å" /> '''Structure of the ste...) |
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| - | [[Image:1yp0.gif|left|200px]]<br /><applet load="1yp0" size=" | + | [[Image:1yp0.gif|left|200px]]<br /><applet load="1yp0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yp0, resolution 1.50Å" /> | caption="1yp0, resolution 1.50Å" /> | ||
'''Structure of the steroidogenic factor-1 ligand binding domain bound to phospholipid and a SHP peptide motif'''<br /> | '''Structure of the steroidogenic factor-1 ligand binding domain bound to phospholipid and a SHP peptide motif'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The orphan nuclear receptor steroidogenic factor 1 (SF-1) regulates the | + | The orphan nuclear receptor steroidogenic factor 1 (SF-1) regulates the differentiation and function of endocrine glands. Although SF-1 is constitutively active in cell-based assays, it is not known whether this transcriptional activity is modulated by ligands. Here, we describe the 1.5 angstroms crystal structure of the SF-1 ligand binding domain in complex with an LXXLL motif from a coregulator protein. The structure reveals the presence of a phospholipid ligand in a surprisingly large pocket (approximately 1600 angstroms3), with the receptor adopting the canonical active conformation. The bound phospholipid is readily exchanged and modulates SF-1 interactions with coactivators. Mutations designed to reduce the size of the SF-1 pocket or to disrupt hydrogen bonds with the phospholipid abolish SF-1/coactivator interactions and significantly reduce SF-1 transcriptional activity. These findings provide evidence that SF-1 is regulated by endogenous ligands and suggest an unexpected relationship between phospholipids and endocrine development and function. |
==About this Structure== | ==About this Structure== | ||
| - | 1YP0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with PEF as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1YP0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=PEF:'>PEF</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YP0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: steroidogenic factor-1]] | [[Category: steroidogenic factor-1]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:34 2008'' |
Revision as of 14:07, 21 February 2008
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Structure of the steroidogenic factor-1 ligand binding domain bound to phospholipid and a SHP peptide motif
Overview
The orphan nuclear receptor steroidogenic factor 1 (SF-1) regulates the differentiation and function of endocrine glands. Although SF-1 is constitutively active in cell-based assays, it is not known whether this transcriptional activity is modulated by ligands. Here, we describe the 1.5 angstroms crystal structure of the SF-1 ligand binding domain in complex with an LXXLL motif from a coregulator protein. The structure reveals the presence of a phospholipid ligand in a surprisingly large pocket (approximately 1600 angstroms3), with the receptor adopting the canonical active conformation. The bound phospholipid is readily exchanged and modulates SF-1 interactions with coactivators. Mutations designed to reduce the size of the SF-1 pocket or to disrupt hydrogen bonds with the phospholipid abolish SF-1/coactivator interactions and significantly reduce SF-1 transcriptional activity. These findings provide evidence that SF-1 is regulated by endogenous ligands and suggest an unexpected relationship between phospholipids and endocrine development and function.
About this Structure
1YP0 is a Protein complex structure of sequences from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Crystallographic identification and functional characterization of phospholipids as ligands for the orphan nuclear receptor steroidogenic factor-1., Li Y, Choi M, Cavey G, Daugherty J, Suino K, Kovach A, Bingham NC, Kliewer SA, Xu HE, Mol Cell. 2005 Feb 18;17(4):491-502. PMID:15721253
Page seeded by OCA on Thu Feb 21 16:07:34 2008
