1yqt
From Proteopedia
(New page: 200px<br /> <applet load="1yqt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yqt, resolution 1.9Å" /> '''RNase-L Inhibitor'''...) |
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| - | [[Image:1yqt.gif|left|200px]]<br /> | + | [[Image:1yqt.gif|left|200px]]<br /><applet load="1yqt" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1yqt" size=" | + | |
caption="1yqt, resolution 1.9Å" /> | caption="1yqt, resolution 1.9Å" /> | ||
'''RNase-L Inhibitor'''<br /> | '''RNase-L Inhibitor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The ABC ATPase RNase-L inhibitor (RLI) emerges as a key enzyme in ribosome | + | The ABC ATPase RNase-L inhibitor (RLI) emerges as a key enzyme in ribosome biogenesis, formation of translation preinitiation complexes, and assembly of HIV capsids. To help reveal the structural mechanism of RLI, we determined the Mg2+-ADP bound crystal structure of the twin cassette ATPase of P. furiosus RLI at 1.9 A resolution and analyzed functional motifs in yeast in vivo. RLI shows similarities but also differences to known ABC enzyme structures. Twin nucleotide binding domains (NBD1 and NBD2) are arranged to form two composite active sites in their interface cleft, indicating they undergo the ATP-driven clamp-like motion of the NBDs of ABC transporters. An unusual "hinge" domain along the NBD1:NBD2 interface provides a frame for association and possibly ATP-driven conformational changes of the NBDs. Our results establish a first structural basis for ABC domain heterodimers and suggest that RLI may act as mechanochemical enzyme in ribosome and HIV capsid biogenesis. |
==About this Structure== | ==About this Structure== | ||
| - | 1YQT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1YQT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YQT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Buttner, K.]] | [[Category: Buttner, K.]] | ||
| - | [[Category: Hopfner, K | + | [[Category: Hopfner, K P.]] |
| - | [[Category: Jansen, R | + | [[Category: Jansen, R P.]] |
[[Category: Karcher, A.]] | [[Category: Karcher, A.]] | ||
[[Category: Martens, B.]] | [[Category: Martens, B.]] | ||
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[[Category: ribosome biogenesis]] | [[Category: ribosome biogenesis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:08:07 2008'' |
Revision as of 14:08, 21 February 2008
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RNase-L Inhibitor
Overview
The ABC ATPase RNase-L inhibitor (RLI) emerges as a key enzyme in ribosome biogenesis, formation of translation preinitiation complexes, and assembly of HIV capsids. To help reveal the structural mechanism of RLI, we determined the Mg2+-ADP bound crystal structure of the twin cassette ATPase of P. furiosus RLI at 1.9 A resolution and analyzed functional motifs in yeast in vivo. RLI shows similarities but also differences to known ABC enzyme structures. Twin nucleotide binding domains (NBD1 and NBD2) are arranged to form two composite active sites in their interface cleft, indicating they undergo the ATP-driven clamp-like motion of the NBDs of ABC transporters. An unusual "hinge" domain along the NBD1:NBD2 interface provides a frame for association and possibly ATP-driven conformational changes of the NBDs. Our results establish a first structural basis for ABC domain heterodimers and suggest that RLI may act as mechanochemical enzyme in ribosome and HIV capsid biogenesis.
About this Structure
1YQT is a Single protein structure of sequence from Pyrococcus furiosus with and as ligands. Full crystallographic information is available from OCA.
Reference
X-ray structure of RLI, an essential twin cassette ABC ATPase involved in ribosome biogenesis and HIV capsid assembly., Karcher A, Buttner K, Martens B, Jansen RP, Hopfner KP, Structure. 2005 Apr;13(4):649-59. PMID:15837203
Page seeded by OCA on Thu Feb 21 16:08:07 2008
