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1ysy
From Proteopedia
(New page: 200px<br /><applet load="1ysy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ysy" /> '''NMR Structure of the nonstructural Protein 7...) |
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| - | [[Image:1ysy.gif|left|200px]]<br /><applet load="1ysy" size=" | + | [[Image:1ysy.gif|left|200px]]<br /><applet load="1ysy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ysy" /> | caption="1ysy" /> | ||
'''NMR Structure of the nonstructural Protein 7 (nsP7) from the SARS CoronaVirus'''<br /> | '''NMR Structure of the nonstructural Protein 7 (nsP7) from the SARS CoronaVirus'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Here, we report the three-dimensional structure of severe acute | + | Here, we report the three-dimensional structure of severe acute respiratory syndrome coronavirus (SARS-CoV) nsP7, a component of the SARS-CoV replicase polyprotein. The coronavirus replicase carries out regulatory tasks involved in the maintenance, transcription, and replication of the coronavirus genome. nsP7 was found to assume a compact architecture in solution, which is comprised primarily of helical secondary structures. Three helices (alpha2 to alpha4) form a flat up-down-up antiparallel alpha-helix sheet. The N-terminal segment of residues 1 to 22, containing two turns of alpha-helix and one turn of 3(10)-helix, is packed across the surface of alpha2 and alpha3 in the helix sheet, with the alpha-helical region oriented at a 60 degrees angle relative to alpha2 and alpha3. The surface charge distribution is pronouncedly asymmetrical, with the flat surface of the helical sheet showing a large negatively charged region adjacent to a large hydrophobic patch and the opposite side containing a positively charged groove that extends along the helix alpha1. Each of these three areas is thus implicated as a potential site for protein-protein interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 1YSY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http:// | + | 1YSY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YSY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Herrmann, T.]] | [[Category: Herrmann, T.]] | ||
| - | [[Category: JCSG, Joint | + | [[Category: JCSG, Joint Center for Structural Genomics.]] |
| - | [[Category: Johnson, M | + | [[Category: Johnson, M A.]] |
[[Category: Kuhn, P.]] | [[Category: Kuhn, P.]] | ||
[[Category: Peti, W.]] | [[Category: Peti, W.]] | ||
| - | [[Category: Stevens, R | + | [[Category: Stevens, R C.]] |
[[Category: Wuthrich, K.]] | [[Category: Wuthrich, K.]] | ||
[[Category: helix bundle]] | [[Category: helix bundle]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:08:46 2008'' |
Revision as of 14:08, 21 February 2008
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NMR Structure of the nonstructural Protein 7 (nsP7) from the SARS CoronaVirus
Overview
Here, we report the three-dimensional structure of severe acute respiratory syndrome coronavirus (SARS-CoV) nsP7, a component of the SARS-CoV replicase polyprotein. The coronavirus replicase carries out regulatory tasks involved in the maintenance, transcription, and replication of the coronavirus genome. nsP7 was found to assume a compact architecture in solution, which is comprised primarily of helical secondary structures. Three helices (alpha2 to alpha4) form a flat up-down-up antiparallel alpha-helix sheet. The N-terminal segment of residues 1 to 22, containing two turns of alpha-helix and one turn of 3(10)-helix, is packed across the surface of alpha2 and alpha3 in the helix sheet, with the alpha-helical region oriented at a 60 degrees angle relative to alpha2 and alpha3. The surface charge distribution is pronouncedly asymmetrical, with the flat surface of the helical sheet showing a large negatively charged region adjacent to a large hydrophobic patch and the opposite side containing a positively charged groove that extends along the helix alpha1. Each of these three areas is thus implicated as a potential site for protein-protein interactions.
About this Structure
1YSY is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.
Reference
Structural genomics of the severe acute respiratory syndrome coronavirus: nuclear magnetic resonance structure of the protein nsP7., Peti W, Johnson MA, Herrmann T, Neuman BW, Buchmeier MJ, Nelson M, Joseph J, Page R, Stevens RC, Kuhn P, Wuthrich K, J Virol. 2005 Oct;79(20):12905-13. PMID:16188992
Page seeded by OCA on Thu Feb 21 16:08:46 2008
Categories: Human sars coronavirus | Single protein | Herrmann, T. | JCSG, Joint Center for Structural Genomics. | Johnson, M A. | Kuhn, P. | Peti, W. | Stevens, R C. | Wuthrich, K. | Helix bundle | Jcsg | Joint center for structural genomics | Protein structure initiative | Psi | Structural genomics
