1ytv
From Proteopedia
(New page: 200px<br /> <applet load="1ytv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ytv, resolution 1.80Å" /> '''Maltose-binding pro...) |
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| - | [[Image:1ytv.gif|left|200px]]<br /> | + | [[Image:1ytv.gif|left|200px]]<br /><applet load="1ytv" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ytv" size=" | + | |
caption="1ytv, resolution 1.80Å" /> | caption="1ytv, resolution 1.80Å" /> | ||
'''Maltose-binding protein fusion to a C-terminal fragment of the V1a vasopressin receptor'''<br /> | '''Maltose-binding protein fusion to a C-terminal fragment of the V1a vasopressin receptor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The V1 vascular vasopressin receptor (V1R) is a G-protein-coupled receptor | + | The V1 vascular vasopressin receptor (V1R) is a G-protein-coupled receptor (GPCR) involved in the regulation of body-fluid osmolality, blood volume and blood pressure. Signal transduction is mediated by the third intracellular loop of this seven-transmembrane protein as well as by the C-terminal cytoplasmic segment. A chimera of the maltose-binding protein (MBP) and the C-terminal segment of V1R has been cloned, expressed, purified and crystallized. The crystals belong to space group P2(1), with unit-cell parameters a = 51.10, b = 66.56, c = 115.72 A, beta = 95.99 degrees. The 1.8 A crystal structure reveals the conformation of MBP and part of the linker region of this chimera, with the C-terminal segment being unstructured. This may reflect a conformational plasticity in the C-terminal segment that may be necessary for proper function of V1R. |
==About this Structure== | ==About this Structure== | ||
| - | 1YTV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1YTV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MAL:'>MAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTV OCA]. |
==Reference== | ==Reference== | ||
| - | A C-terminal segment of the V1R vasopressin receptor is unstructured in the crystal structure of its chimera with the maltose-binding protein., Adikesavan NV, Mahmood SS, Stanley N, Xu Z, Wu N, Thibonnier M, Shoham M, Acta | + | A C-terminal segment of the V1R vasopressin receptor is unstructured in the crystal structure of its chimera with the maltose-binding protein., Adikesavan NV, Mahmood SS, Stanley N, Xu Z, Wu N, Thibonnier M, Shoham M, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt, 4):341-5. Epub 2005 Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511036 16511036] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Adikesavan, N | + | [[Category: Adikesavan, N V]] |
| - | [[Category: Mahmood, S | + | [[Category: Mahmood, S S.]] |
[[Category: Shoham, M.]] | [[Category: Shoham, M.]] | ||
[[Category: Stanley,, S.]] | [[Category: Stanley,, S.]] | ||
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[[Category: vasopressin; receptor; gpcr; fusion protein; maltose-binding protein]] | [[Category: vasopressin; receptor; gpcr; fusion protein; maltose-binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:02 2008'' |
Revision as of 14:09, 21 February 2008
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Maltose-binding protein fusion to a C-terminal fragment of the V1a vasopressin receptor
Overview
The V1 vascular vasopressin receptor (V1R) is a G-protein-coupled receptor (GPCR) involved in the regulation of body-fluid osmolality, blood volume and blood pressure. Signal transduction is mediated by the third intracellular loop of this seven-transmembrane protein as well as by the C-terminal cytoplasmic segment. A chimera of the maltose-binding protein (MBP) and the C-terminal segment of V1R has been cloned, expressed, purified and crystallized. The crystals belong to space group P2(1), with unit-cell parameters a = 51.10, b = 66.56, c = 115.72 A, beta = 95.99 degrees. The 1.8 A crystal structure reveals the conformation of MBP and part of the linker region of this chimera, with the C-terminal segment being unstructured. This may reflect a conformational plasticity in the C-terminal segment that may be necessary for proper function of V1R.
About this Structure
1YTV is a Protein complex structure of sequences from Escherichia coli and Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
A C-terminal segment of the V1R vasopressin receptor is unstructured in the crystal structure of its chimera with the maltose-binding protein., Adikesavan NV, Mahmood SS, Stanley N, Xu Z, Wu N, Thibonnier M, Shoham M, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt, 4):341-5. Epub 2005 Mar 24. PMID:16511036
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