1yvu
From Proteopedia
(New page: 200px<br /><applet load="1yvu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yvu, resolution 2.90Å" /> '''Crystal structure of...) |
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| - | [[Image:1yvu.gif|left|200px]]<br /><applet load="1yvu" size=" | + | [[Image:1yvu.gif|left|200px]]<br /><applet load="1yvu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yvu, resolution 2.90Å" /> | caption="1yvu, resolution 2.90Å" /> | ||
'''Crystal structure of A. aeolicus Argonaute'''<br /> | '''Crystal structure of A. aeolicus Argonaute'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Argonaute (Ago) proteins constitute a key component of the RNA-induced | + | Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC. |
==About this Structure== | ==About this Structure== | ||
| - | 1YVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1YVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YVU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Aquifex aeolicus vf5]] | [[Category: Aquifex aeolicus vf5]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chen, H | + | [[Category: Chen, H Y.]] |
[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
[[Category: Kuryavyi, V.]] | [[Category: Kuryavyi, V.]] | ||
| - | [[Category: Ma, J | + | [[Category: Ma, J B.]] |
[[Category: Meister, G.]] | [[Category: Meister, G.]] | ||
| - | [[Category: Patel, D | + | [[Category: Patel, D J.]] |
[[Category: Pei, Y.]] | [[Category: Pei, Y.]] | ||
[[Category: Tuschl, T.]] | [[Category: Tuschl, T.]] | ||
| - | [[Category: Yuan, Y | + | [[Category: Yuan, Y R.]] |
[[Category: Zhadina, M.]] | [[Category: Zhadina, M.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: rnase h fold]] | [[Category: rnase h fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:42 2008'' |
Revision as of 14:09, 21 February 2008
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Crystal structure of A. aeolicus Argonaute
Overview
Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC.
About this Structure
1YVU is a Single protein structure of sequence from Aquifex aeolicus vf5 with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage., Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ, Mol Cell. 2005 Aug 5;19(3):405-19. PMID:16061186
Page seeded by OCA on Thu Feb 21 16:09:42 2008
Categories: Aquifex aeolicus vf5 | Single protein | Chen, H Y. | Dauter, Z. | Kuryavyi, V. | Ma, J B. | Meister, G. | Patel, D J. | Pei, Y. | Tuschl, T. | Yuan, Y R. | Zhadina, M. | CA | Rnase h fold
