1ywf
From Proteopedia
(New page: 200px<br /><applet load="1ywf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ywf, resolution 1.71Å" /> '''Crystal Structure of...) |
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| - | [[Image:1ywf.gif|left|200px]]<br /><applet load="1ywf" size=" | + | [[Image:1ywf.gif|left|200px]]<br /><applet load="1ywf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ywf, resolution 1.71Å" /> | caption="1ywf, resolution 1.71Å" /> | ||
'''Crystal Structure of Mycobacterium Tuberculosis Protein Tyrosine Phosphatase PtpB'''<br /> | '''Crystal Structure of Mycobacterium Tuberculosis Protein Tyrosine Phosphatase PtpB'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Intracellular pathogenic bacteria manipulate host signal transduction | + | Intracellular pathogenic bacteria manipulate host signal transduction pathways to facilitate infection. Mycobacterium tuberculosis protein tyrosine phosphatases (PTPs) PtpA and PtpB are thought to be secreted into host cells and interfere with unidentified signals. To illuminate the mechanisms of regulation and substrate recognition, we determined the 1.7 A resolution crystal structure of PtpB in complex with the product phosphate. The protein adopts a simplified PTP fold, which combines features of the conventional PTPs and dual-specificity phosphatases. PtpB shows two unusual elaborations--a disordered, acidic loop and a flexible, two-helix lid that covers the active site--that are specific to mycobacterial orthologs. Biochemical studies suggest that substrate mimicry in the lid may protect the phosphatase from oxidative inactivation. The insertion and deletion of large structural elements in PtpB suggest that, outside the active site module, the PTP family is under unusual selective pressure that promotes changes in overall structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1YWF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1YWF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Alber, T.]] | [[Category: Alber, T.]] | ||
[[Category: Grundner, C.]] | [[Category: Grundner, C.]] | ||
| - | [[Category: Ng, H | + | [[Category: Ng, H L.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: four stranded parallel beta sheet with flanking helices]] | [[Category: four stranded parallel beta sheet with flanking helices]] | ||
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[[Category: tbsgc]] | [[Category: tbsgc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:46 2008'' |
Revision as of 14:09, 21 February 2008
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Crystal Structure of Mycobacterium Tuberculosis Protein Tyrosine Phosphatase PtpB
Overview
Intracellular pathogenic bacteria manipulate host signal transduction pathways to facilitate infection. Mycobacterium tuberculosis protein tyrosine phosphatases (PTPs) PtpA and PtpB are thought to be secreted into host cells and interfere with unidentified signals. To illuminate the mechanisms of regulation and substrate recognition, we determined the 1.7 A resolution crystal structure of PtpB in complex with the product phosphate. The protein adopts a simplified PTP fold, which combines features of the conventional PTPs and dual-specificity phosphatases. PtpB shows two unusual elaborations--a disordered, acidic loop and a flexible, two-helix lid that covers the active site--that are specific to mycobacterial orthologs. Biochemical studies suggest that substrate mimicry in the lid may protect the phosphatase from oxidative inactivation. The insertion and deletion of large structural elements in PtpB suggest that, outside the active site module, the PTP family is under unusual selective pressure that promotes changes in overall structure.
About this Structure
1YWF is a Single protein structure of sequence from Mycobacterium tuberculosis with as ligand. Full crystallographic information is available from OCA.
Reference
Mycobacterium tuberculosis protein tyrosine phosphatase PtpB structure reveals a diverged fold and a buried active site., Grundner C, Ng HL, Alber T, Structure. 2005 Nov;13(11):1625-34. PMID:16271885
Page seeded by OCA on Thu Feb 21 16:09:46 2008
Categories: Mycobacterium tuberculosis | Single protein | Alber, T. | Grundner, C. | Ng, H L. | TBSGC, TB Structural Genomics Consortium. | PO4 | Four stranded parallel beta sheet with flanking helices | Protein structure initiative | Psi | Structural genomics | Tb structural genomics consortium | Tbsgc
