This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1yx4
From Proteopedia
(New page: 200px<br /> <applet load="1yx4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yx4" /> '''Structure of S5a bound to monoubiquitin pro...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1yx4.gif|left|200px]]<br /> | + | [[Image:1yx4.gif|left|200px]]<br /><applet load="1yx4" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1yx4" size=" | + | |
caption="1yx4" /> | caption="1yx4" /> | ||
'''Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition'''<br /> | '''Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ubiquitin is a key regulatory molecule in diverse cellular events. How | + | Ubiquitin is a key regulatory molecule in diverse cellular events. How cells determine the outcome of ubiquitylation remains unclear; however, a likely determinant is the specificity of ubiquitin receptor proteins for polyubiquitin chains of certain length and linkage. Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ubiquitin-binding mechanisms and provide a rationale for their unique specificities for different ubiquitin-like domains. Furthermore, we provide direct evidence that S5a (196-306) binds either K63-linked or K48-linked polyubiquitin, and in both cases prefers longer chains. On the basis of these results we present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin. |
==About this Structure== | ==About this Structure== | ||
| - | 1YX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1YX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YX4 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 13: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Walters, K | + | [[Category: Walters, K J.]] |
[[Category: Wang, Q.]] | [[Category: Wang, Q.]] | ||
[[Category: Young, P.]] | [[Category: Young, P.]] | ||
| Line 23: | Line 22: | ||
[[Category: uim]] | [[Category: uim]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:59 2008'' |
Revision as of 14:10, 21 February 2008
|
Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition
Overview
Ubiquitin is a key regulatory molecule in diverse cellular events. How cells determine the outcome of ubiquitylation remains unclear; however, a likely determinant is the specificity of ubiquitin receptor proteins for polyubiquitin chains of certain length and linkage. Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ubiquitin-binding mechanisms and provide a rationale for their unique specificities for different ubiquitin-like domains. Furthermore, we provide direct evidence that S5a (196-306) binds either K63-linked or K48-linked polyubiquitin, and in both cases prefers longer chains. On the basis of these results we present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin.
About this Structure
1YX4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition., Wang Q, Young P, Walters KJ, J Mol Biol. 2005 May 6;348(3):727-39. PMID:15826667
Page seeded by OCA on Thu Feb 21 16:09:59 2008
Categories: Homo sapiens | Single protein | Walters, K J. | Wang, Q. | Young, P. | Nmr | Polyubiquitin | Proteasome | S5a | Uim
