1yzc
From Proteopedia
(New page: 200px<br /><applet load="1yzc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yzc" /> '''The solution structure of a redesigned apocy...) |
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| - | [[Image:1yzc.gif|left|200px]]<br /><applet load="1yzc" size=" | + | [[Image:1yzc.gif|left|200px]]<br /><applet load="1yzc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yzc" /> | caption="1yzc" /> | ||
'''The solution structure of a redesigned apocytochrome B562 (Rd-apocyt b562) with the N- and a part of the C-terminal helices unfolded'''<br /> | '''The solution structure of a redesigned apocytochrome B562 (Rd-apocyt b562) with the N- and a part of the C-terminal helices unfolded'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Using native-state hydrogen-exchange-directed protein engineering and | + | Using native-state hydrogen-exchange-directed protein engineering and multidimensional NMR, we determined the high-resolution structure (rms deviation, 1.1 angstroms) for an intermediate of the four-helix bundle protein: Rd-apocytochrome b562. The intermediate has the N-terminal helix and a part of the C-terminal helix unfolded. In earlier studies, we also solved the structures of two other folding intermediates for the same protein: one with the N-terminal helix alone unfolded and the other with a reorganized hydrophobic core. Together, these structures provide a description of a protein folding pathway with multiple intermediates at atomic resolution. The two general features for the intermediates are (i) native-like backbone topology and (ii) nonnative side-chain interactions. These results have implications for important issues in protein folding studies, including large-scale conformation search, -value analysis, and computer simulations. |
==About this Structure== | ==About this Structure== | ||
| - | 1YZC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1YZC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: BSGC, Berkeley | + | [[Category: BSGC, Berkeley Structural Genomics Center.]] |
[[Category: Bai, Y.]] | [[Category: Bai, Y.]] | ||
[[Category: Feng, H.]] | [[Category: Feng, H.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:38 2008'' |
Revision as of 14:10, 21 February 2008
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The solution structure of a redesigned apocytochrome B562 (Rd-apocyt b562) with the N- and a part of the C-terminal helices unfolded
Overview
Using native-state hydrogen-exchange-directed protein engineering and multidimensional NMR, we determined the high-resolution structure (rms deviation, 1.1 angstroms) for an intermediate of the four-helix bundle protein: Rd-apocytochrome b562. The intermediate has the N-terminal helix and a part of the C-terminal helix unfolded. In earlier studies, we also solved the structures of two other folding intermediates for the same protein: one with the N-terminal helix alone unfolded and the other with a reorganized hydrophobic core. Together, these structures provide a description of a protein folding pathway with multiple intermediates at atomic resolution. The two general features for the intermediates are (i) native-like backbone topology and (ii) nonnative side-chain interactions. These results have implications for important issues in protein folding studies, including large-scale conformation search, -value analysis, and computer simulations.
About this Structure
1YZC is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A protein folding pathway with multiple folding intermediates at atomic resolution., Feng H, Zhou Z, Bai Y, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5026-31. Epub 2005 Mar 25. PMID:15793003
Page seeded by OCA on Thu Feb 21 16:10:38 2008
Categories: Homo sapiens | Protein complex | BSGC, Berkeley Structural Genomics Center. | Bai, Y. | Feng, H. | Zhou, Z. | Berkeley structural genomics center | Bsgc | Folding intermediates | Native-state hydrogen exchange | Nmr | Protein engineering | Protein structure | Protein structure initiative | Psi | Structural genomics
