1z00
From Proteopedia
(New page: 200px<br /> <applet load="1z00" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z00" /> '''Solution structure of the C-terminal domain...) |
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'''Solution structure of the C-terminal domain of ERCC1 complexed with the C-terminal domain of XPF'''<br /> | '''Solution structure of the C-terminal domain of ERCC1 complexed with the C-terminal domain of XPF'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The human ERCC1/XPF complex is a structure-specific endonuclease with | + | The human ERCC1/XPF complex is a structure-specific endonuclease with defined polarity that participates in multiple DNA repair pathways. We report the heterodimeric structure of the C-terminal domains of both proteins responsible for ERCC1/XPF complex formation. Both domains exhibit the double helix-hairpin-helix motif (HhH)2, and they are related by a pseudo-2-fold symmetry axis. In the XPF domain, the hairpin of the second motif is replaced by a short turn. The ERCC1 domain folds properly only in the presence of the XPF domain, which implies a role for XPF as a scaffold for the folding of ERCC1. The intersubunit interactions are largely hydrophobic in nature. NMR titration data show that only the ERCC1 domain of the ERCC1/XPF complex is involved in DNA binding. On the basis of these findings, we propose a model for the targeting of XPF nuclease via ERCC1-mediated interactions in the context of nucleotide excision repair. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z00 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1Z00 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z00 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Das, D.]] | [[Category: Das, D.]] | ||
[[Category: Folkers, G.]] | [[Category: Folkers, G.]] | ||
| - | [[Category: Hoeijmakers, J | + | [[Category: Hoeijmakers, J H.J.]] |
| - | [[Category: Jaspers, N | + | [[Category: Jaspers, N G.J.]] |
[[Category: Kaptein, R.]] | [[Category: Kaptein, R.]] | ||
[[Category: Odijk, H.]] | [[Category: Odijk, H.]] | ||
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[[Category: helix-hairpin-helix]] | [[Category: helix-hairpin-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:49 2008'' |
Revision as of 14:10, 21 February 2008
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Solution structure of the C-terminal domain of ERCC1 complexed with the C-terminal domain of XPF
Contents |
Overview
The human ERCC1/XPF complex is a structure-specific endonuclease with defined polarity that participates in multiple DNA repair pathways. We report the heterodimeric structure of the C-terminal domains of both proteins responsible for ERCC1/XPF complex formation. Both domains exhibit the double helix-hairpin-helix motif (HhH)2, and they are related by a pseudo-2-fold symmetry axis. In the XPF domain, the hairpin of the second motif is replaced by a short turn. The ERCC1 domain folds properly only in the presence of the XPF domain, which implies a role for XPF as a scaffold for the folding of ERCC1. The intersubunit interactions are largely hydrophobic in nature. NMR titration data show that only the ERCC1 domain of the ERCC1/XPF complex is involved in DNA binding. On the basis of these findings, we propose a model for the targeting of XPF nuclease via ERCC1-mediated interactions in the context of nucleotide excision repair.
Disease
Known diseases associated with this structure: Cerebrooculofacioskeletal syndrome 4 OMIM:[126380], XFE progeroid syndrome OMIM:[133520], Xeroderma pigmentosum, group F OMIM:[133520]
About this Structure
1Z00 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of the human ERCC1/XPF interaction domains reveals a complementary role for the two proteins in nucleotide excision repair., Tripsianes K, Folkers G, Ab E, Das D, Odijk H, Jaspers NG, Hoeijmakers JH, Kaptein R, Boelens R, Structure. 2005 Dec;13(12):1849-58. PMID:16338413
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