1z6a
From Proteopedia
(New page: 200px<br /><applet load="1z6a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z6a, resolution 3.0Å" /> '''Sulfolobus solfataric...) |
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| - | [[Image:1z6a.gif|left|200px]]<br /><applet load="1z6a" size=" | + | [[Image:1z6a.gif|left|200px]]<br /><applet load="1z6a" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z6a, resolution 3.0Å" /> | caption="1z6a, resolution 3.0Å" /> | ||
'''Sulfolobus solfataricus SWI2/SNF2 ATPase core domain'''<br /> | '''Sulfolobus solfataricus SWI2/SNF2 ATPase core domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | SWI2/SNF2 ATPases remodel chromatin or other DNA:protein complexes by a | + | SWI2/SNF2 ATPases remodel chromatin or other DNA:protein complexes by a poorly understood mechanism that involves ATP-dependent DNA translocation and generation of superhelical torsion. Crystal structures of a dsDNA-translocating SWI2/SNF2 ATPase core from Sulfolobus solfataricus reveal two helical SWI2/SNF2 specific subdomains, fused to a DExx box helicase-related ATPase core. Fully base paired duplex DNA binds along a central cleft via both minor groove strands, indicating that SWI2/SNF2 ATPases travel along the dsDNA minor groove without strand separation. A structural switch, linking DNA binding and the active site DExx motif, may account for the stimulation of ATPase activity by dsDNA. Our results suggest that torque in remodeling processes is generated by an ATP-driven screw motion of DNA along the active site cleft. The structures also redefine SWI2/SNF2 functional motifs and uncover unexpected structural correlation of mutations in Cockayne and X-linked mental retardation syndromes. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2] with HG and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1Z6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z6A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Duerr, H.]] | [[Category: Duerr, H.]] | ||
[[Category: Hickmann, V.]] | [[Category: Hickmann, V.]] | ||
| - | [[Category: Hopfner, K | + | [[Category: Hopfner, K P.]] |
[[Category: Koerner, C.]] | [[Category: Koerner, C.]] | ||
[[Category: Mueller, M.]] | [[Category: Mueller, M.]] | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:25 2008'' |
Revision as of 14:12, 21 February 2008
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Sulfolobus solfataricus SWI2/SNF2 ATPase core domain
Overview
SWI2/SNF2 ATPases remodel chromatin or other DNA:protein complexes by a poorly understood mechanism that involves ATP-dependent DNA translocation and generation of superhelical torsion. Crystal structures of a dsDNA-translocating SWI2/SNF2 ATPase core from Sulfolobus solfataricus reveal two helical SWI2/SNF2 specific subdomains, fused to a DExx box helicase-related ATPase core. Fully base paired duplex DNA binds along a central cleft via both minor groove strands, indicating that SWI2/SNF2 ATPases travel along the dsDNA minor groove without strand separation. A structural switch, linking DNA binding and the active site DExx motif, may account for the stimulation of ATPase activity by dsDNA. Our results suggest that torque in remodeling processes is generated by an ATP-driven screw motion of DNA along the active site cleft. The structures also redefine SWI2/SNF2 functional motifs and uncover unexpected structural correlation of mutations in Cockayne and X-linked mental retardation syndromes.
About this Structure
1Z6A is a Single protein structure of sequence from Sulfolobus solfataricus p2 with and as ligands. Full crystallographic information is available from OCA.
Reference
X-ray structures of the Sulfolobus solfataricus SWI2/SNF2 ATPase core and its complex with DNA., Durr H, Korner C, Muller M, Hickmann V, Hopfner KP, Cell. 2005 May 6;121(3):363-73. PMID:15882619
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