1z64
From Proteopedia
(New page: 200px<br /><applet load="1z64" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z64" /> '''NMR Solution Structure of Pleurocidin in DPC...) |
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| - | [[Image:1z64.gif|left|200px]]<br /><applet load="1z64" size=" | + | [[Image:1z64.gif|left|200px]]<br /><applet load="1z64" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z64" /> | caption="1z64" /> | ||
'''NMR Solution Structure of Pleurocidin in DPC Micelles'''<br /> | '''NMR Solution Structure of Pleurocidin in DPC Micelles'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pleurocidin is an antimicrobial peptide that was isolated from the mucus | + | Pleurocidin is an antimicrobial peptide that was isolated from the mucus membranes of winter flounder (Pseudopleuronectes americanus) and contributes to the initial stages of defense against bacterial infection. From NMR structural studies with the uniformly (15)N-labeled peptide, a structure of pleurocidin was determined to be in a random coil conformation in aqueous solution whereas it assumes an alpha-helical structure in TFE and in dodecylphosphocholine (DPC) micelles. From (15)N relaxation studies, the helix is a rigid structure in the membrane-mimicking environment. Strong NOESY cross-peaks from the pleurocidin to the aliphatic chain on DPC confirm that pleurocidin is contained within the DPC micelle and not associated with the surface of the micelle. From diffusion studies it was determined that each micelle contains at least two pleurocidin molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z64 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudopleuronectes_americanus Pseudopleuronectes americanus] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1Z64 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudopleuronectes_americanus Pseudopleuronectes americanus] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z64 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Burton, I.]] | [[Category: Burton, I.]] | ||
| - | [[Category: Douglas, S | + | [[Category: Douglas, S E.]] |
| - | [[Category: Jakeman, D | + | [[Category: Jakeman, D L.]] |
| - | [[Category: Mattatall, N | + | [[Category: Mattatall, N R.]] |
| - | [[Category: Syvitski, R | + | [[Category: Syvitski, R T.]] |
[[Category: NH2]] | [[Category: NH2]] | ||
[[Category: helix; micelle]] | [[Category: helix; micelle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:27 2008'' |
Revision as of 14:12, 21 February 2008
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NMR Solution Structure of Pleurocidin in DPC Micelles
Overview
Pleurocidin is an antimicrobial peptide that was isolated from the mucus membranes of winter flounder (Pseudopleuronectes americanus) and contributes to the initial stages of defense against bacterial infection. From NMR structural studies with the uniformly (15)N-labeled peptide, a structure of pleurocidin was determined to be in a random coil conformation in aqueous solution whereas it assumes an alpha-helical structure in TFE and in dodecylphosphocholine (DPC) micelles. From (15)N relaxation studies, the helix is a rigid structure in the membrane-mimicking environment. Strong NOESY cross-peaks from the pleurocidin to the aliphatic chain on DPC confirm that pleurocidin is contained within the DPC micelle and not associated with the surface of the micelle. From diffusion studies it was determined that each micelle contains at least two pleurocidin molecules.
About this Structure
1Z64 is a Single protein structure of sequence from Pseudopleuronectes americanus with as ligand. Full crystallographic information is available from OCA.
Reference
Structural characterization of the antimicrobial peptide pleurocidin from winter flounder., Syvitski RT, Burton I, Mattatall NR, Douglas SE, Jakeman DL, Biochemistry. 2005 May 17;44(19):7282-93. PMID:15882067
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