1z7h
From Proteopedia
(New page: 200px<br /><applet load="1z7h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z7h, resolution 2.30Å" /> '''2.3 Angstrom crystal...) |
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| - | [[Image:1z7h.gif|left|200px]]<br /><applet load="1z7h" size=" | + | [[Image:1z7h.gif|left|200px]]<br /><applet load="1z7h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z7h, resolution 2.30Å" /> | caption="1z7h, resolution 2.30Å" /> | ||
'''2.3 Angstrom crystal structure of tetanus neurotoxin light chain'''<br /> | '''2.3 Angstrom crystal structure of tetanus neurotoxin light chain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | TeNT is the causative agent of the neuroparalytic disease tetanus. A key | + | TeNT is the causative agent of the neuroparalytic disease tetanus. A key component of TeNT is its light chain, a Zn(2+) endopeptidase that targets SNAREs. Recent structural studies of closely related BoNT endopeptidases indicate that substrate-binding exosites remote from a conserved active site are the primary determinants of substrate specificity. Here we report the 2.3 A X-ray crystal structure of TeNT-LC, determined by combined molecular replacement and MAD phasing. As expected, the overall structure of TeNT-LC is similar to the other known CNT light chain structures, including a conserved thermolysin-like core inserted between structurally distinct amino- and carboxy-terminal regions. Differences between TeNT-LC and the other CNT light chains are mainly limited to surface features such as unique electrostatic potential profiles. An analysis of surface residue conservation reveals a pattern of relatively high variability matching the path of substrate binding around BoNT/A, possibly serving to accommodate the variations in different SNARE targets of the CNT group. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z7H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] Full crystallographic information is available from [http:// | + | 1Z7H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Tentoxilysin]] | [[Category: Tentoxilysin]] | ||
| - | [[Category: Breidenbach, M | + | [[Category: Breidenbach, M A.]] |
| - | [[Category: Brunger, A | + | [[Category: Brunger, A T.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: metalloprotease]] | [[Category: metalloprotease]] | ||
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[[Category: tetanus]] | [[Category: tetanus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:48 2008'' |
Revision as of 14:12, 21 February 2008
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2.3 Angstrom crystal structure of tetanus neurotoxin light chain
Overview
TeNT is the causative agent of the neuroparalytic disease tetanus. A key component of TeNT is its light chain, a Zn(2+) endopeptidase that targets SNAREs. Recent structural studies of closely related BoNT endopeptidases indicate that substrate-binding exosites remote from a conserved active site are the primary determinants of substrate specificity. Here we report the 2.3 A X-ray crystal structure of TeNT-LC, determined by combined molecular replacement and MAD phasing. As expected, the overall structure of TeNT-LC is similar to the other known CNT light chain structures, including a conserved thermolysin-like core inserted between structurally distinct amino- and carboxy-terminal regions. Differences between TeNT-LC and the other CNT light chains are mainly limited to surface features such as unique electrostatic potential profiles. An analysis of surface residue conservation reveals a pattern of relatively high variability matching the path of substrate binding around BoNT/A, possibly serving to accommodate the variations in different SNARE targets of the CNT group.
About this Structure
1Z7H is a Single protein structure of sequence from Clostridium tetani with as ligand. Active as Tentoxilysin, with EC number 3.4.24.68 Full crystallographic information is available from OCA.
Reference
2.3 A crystal structure of tetanus neurotoxin light chain., Breidenbach MA, Brunger AT, Biochemistry. 2005 May 24;44(20):7450-7. PMID:15895988
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