1zc5
From Proteopedia
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'''Structure of the RNA signal essential for translational frameshifting in HIV-1'''<br /> | '''Structure of the RNA signal essential for translational frameshifting in HIV-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Many pathogenic viruses use a programmed -1 translational frameshifting | + | Many pathogenic viruses use a programmed -1 translational frameshifting mechanism to regulate synthesis of their structural and enzymatic proteins. Frameshifting is vital for viral replication. A slippery sequence bound at the ribosomal A and P sites as well as a downstream stimulatory RNA structure are essential for frameshifting. Conflicting data have been reported concerning the structure of the downstream RNA signal in human immunodeficiency virus type 1 (HIV-1). Here, the solution structure of the HIV-1 frameshifting RNA signal was solved by heteronuclear NMR spectroscopy. This structure reveals a long hairpin fold with an internal three-nucleotide bulge. The internal loop introduces a bend between the lower and upper helical regions, a structural feature often seen in frameshifting pseudoknots. The NMR structure correlates with chemical probing data. The upper stem rich in conserved G-C Watson-Crick base-pairs is highly stable, whereas the bulge region and the lower stem are more flexible. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZC5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1ZC5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZC5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gaudin, C.]] | [[Category: Gaudin, C.]] | ||
[[Category: Guittet, E.]] | [[Category: Guittet, E.]] | ||
| - | [[Category: Mazauric, M | + | [[Category: Mazauric, M H.]] |
[[Category: Traikia, M.]] | [[Category: Traikia, M.]] | ||
[[Category: Yoshizawa, S.]] | [[Category: Yoshizawa, S.]] | ||
[[Category: rna bulged helix]] | [[Category: rna bulged helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:14:06 2008'' |
Revision as of 14:14, 21 February 2008
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Structure of the RNA signal essential for translational frameshifting in HIV-1
Overview
Many pathogenic viruses use a programmed -1 translational frameshifting mechanism to regulate synthesis of their structural and enzymatic proteins. Frameshifting is vital for viral replication. A slippery sequence bound at the ribosomal A and P sites as well as a downstream stimulatory RNA structure are essential for frameshifting. Conflicting data have been reported concerning the structure of the downstream RNA signal in human immunodeficiency virus type 1 (HIV-1). Here, the solution structure of the HIV-1 frameshifting RNA signal was solved by heteronuclear NMR spectroscopy. This structure reveals a long hairpin fold with an internal three-nucleotide bulge. The internal loop introduces a bend between the lower and upper helical regions, a structural feature often seen in frameshifting pseudoknots. The NMR structure correlates with chemical probing data. The upper stem rich in conserved G-C Watson-Crick base-pairs is highly stable, whereas the bulge region and the lower stem are more flexible.
About this Structure
1ZC5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the RNA signal essential for translational frameshifting in HIV-1., Gaudin C, Mazauric MH, Traikia M, Guittet E, Yoshizawa S, Fourmy D, J Mol Biol. 2005 Jun 24;349(5):1024-35. PMID:15907937
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