1zcw
From Proteopedia
(New page: 200px<br /><applet load="1zcw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zcw, resolution 2.25Å" /> '''Co-crystal structure...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1zcw.gif|left|200px]]<br /><applet load="1zcw" size=" | + | [[Image:1zcw.gif|left|200px]]<br /><applet load="1zcw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1zcw, resolution 2.25Å" /> | caption="1zcw, resolution 2.25Å" /> | ||
'''Co-crystal structure of Orf2 an aromatic prenyl transferase from Streptomyces sp. strain CL190 complexed with GPP'''<br /> | '''Co-crystal structure of Orf2 an aromatic prenyl transferase from Streptomyces sp. strain CL190 complexed with GPP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The anti-oxidant naphterpin is a natural product containing a | + | The anti-oxidant naphterpin is a natural product containing a polyketide-based aromatic core with an attached 10-carbon geranyl group derived from isoprenoid (terpene) metabolism. Hybrid natural products such as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl) or 15-carbon (farnesyl) isoprenoid chains possess biological activities distinct from their non-prenylated aromatic precursors. These hybrid natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of aromatic prenyltransferases (PTases) responsible for prenyl group attachment have only recently been isolated and characterized. Here we report the gene identification, biochemical characterization and high-resolution X-ray crystal structures of an architecturally novel aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and carbon-oxygen-based prenylation of a diverse collection of hydroxyl-containing aromatic acceptors of synthetic, microbial and plant origin. These crystal structures, coupled with in vitro assays, provide a basis for understanding and potentially manipulating the regio-specific prenylation of aromatic small molecules using this structurally unique family of aromatic PTases. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZCW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with MG, NO3 and GPP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ZCW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NO3:'>NO3</scene> and <scene name='pdbligand=GPP:'>GPP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZCW OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Streptomyces sp.]] | [[Category: Streptomyces sp.]] | ||
[[Category: Kuzuyama, T.]] | [[Category: Kuzuyama, T.]] | ||
| - | [[Category: Noel, J | + | [[Category: Noel, J P.]] |
| - | [[Category: Richard, S | + | [[Category: Richard, S B.]] |
[[Category: GPP]] | [[Category: GPP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 22: | Line 22: | ||
[[Category: pt-barrel]] | [[Category: pt-barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:14:17 2008'' |
Revision as of 14:14, 21 February 2008
|
Co-crystal structure of Orf2 an aromatic prenyl transferase from Streptomyces sp. strain CL190 complexed with GPP
Overview
The anti-oxidant naphterpin is a natural product containing a polyketide-based aromatic core with an attached 10-carbon geranyl group derived from isoprenoid (terpene) metabolism. Hybrid natural products such as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl) or 15-carbon (farnesyl) isoprenoid chains possess biological activities distinct from their non-prenylated aromatic precursors. These hybrid natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of aromatic prenyltransferases (PTases) responsible for prenyl group attachment have only recently been isolated and characterized. Here we report the gene identification, biochemical characterization and high-resolution X-ray crystal structures of an architecturally novel aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and carbon-oxygen-based prenylation of a diverse collection of hydroxyl-containing aromatic acceptors of synthetic, microbial and plant origin. These crystal structures, coupled with in vitro assays, provide a basis for understanding and potentially manipulating the regio-specific prenylation of aromatic small molecules using this structurally unique family of aromatic PTases.
About this Structure
1ZCW is a Protein complex structure of sequences from Streptomyces sp. with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products., Kuzuyama T, Noel JP, Richard SB, Nature. 2005 Jun 16;435(7044):983-7. PMID:15959519
Page seeded by OCA on Thu Feb 21 16:14:17 2008
