1zh6
From Proteopedia
(New page: 200px<br /><applet load="1zh6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zh6, resolution 2.50Å" /> '''Crystal Structure of...) |
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| - | [[Image:1zh6.gif|left|200px]]<br /><applet load="1zh6" size=" | + | [[Image:1zh6.gif|left|200px]]<br /><applet load="1zh6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1zh6, resolution 2.50Å" /> | caption="1zh6, resolution 2.50Å" /> | ||
'''Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine'''<br /> | '''Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZH6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with 4AF and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] Full crystallographic information is available from [http:// | + | 1ZH6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=4AF:'>4AF</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZH6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Tyrosine--tRNA ligase]] | [[Category: Tyrosine--tRNA ligase]] | ||
[[Category: Graziano, J.]] | [[Category: Graziano, J.]] | ||
| - | [[Category: Schultz, P | + | [[Category: Schultz, P G.]] |
[[Category: Spraggon, G.]] | [[Category: Spraggon, G.]] | ||
| - | [[Category: Turner, J | + | [[Category: Turner, J M.]] |
[[Category: 4AF]] | [[Category: 4AF]] | ||
[[Category: BME]] | [[Category: BME]] | ||
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[[Category: unnatural amino acid]] | [[Category: unnatural amino acid]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:15:29 2008'' |
Revision as of 14:15, 21 February 2008
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Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine
Overview
It has been recently shown that orthogonal tRNA/aminoacyl-tRNA synthetase pairs can be evolved to allow genetic incorporation of unnatural amino acids into proteins in both prokaryotes and eukaryotes. Here we describe the crystal structure of an evolved aminoacyl-tRNA synthetase that charges the unnatural amino acid p-acetylphenylalanine. Molecular recognition is due to altered hydrogen bonding and packing interactions with bound substrate that result from changes in both side-chain and backbone conformation.
About this Structure
1ZH6 is a Single protein structure of sequence from Methanocaldococcus jannaschii with and as ligands. Active as Tyrosine--tRNA ligase, with EC number 6.1.1.1 Full crystallographic information is available from OCA.
Reference
Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase., Turner JM, Graziano J, Spraggon G, Schultz PG, J Am Chem Soc. 2005 Nov 2;127(43):14976-7. PMID:16248607
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