1zib
From Proteopedia
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==Overview== | ==Overview== | ||
| - | New crystals of a blue copper protein, pseudoazurin from denitrifier | + | New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013., Inoue T, Nishio N, Kai Y, Harada S, Ohshiro Y, Suzuki S, Kohzuma T, Shidara S, Iwasaki H, J Biochem | + | Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013., Inoue T, Nishio N, Kai Y, Harada S, Ohshiro Y, Suzuki S, Kohzuma T, Shidara S, Iwasaki H, J Biochem. 1993 Dec;114(6):761-2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8138527 8138527] |
[[Category: Achromobacter cycloclastes]] | [[Category: Achromobacter cycloclastes]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:15:49 2008'' |
Revision as of 14:15, 21 February 2008
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REDUCED PSEUDOAZURIN
Overview
New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress.
About this Structure
1ZIB is a Single protein structure of sequence from Achromobacter cycloclastes with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013., Inoue T, Nishio N, Kai Y, Harada S, Ohshiro Y, Suzuki S, Kohzuma T, Shidara S, Iwasaki H, J Biochem. 1993 Dec;114(6):761-2. PMID:8138527
Page seeded by OCA on Thu Feb 21 16:15:49 2008
