We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1zj6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1zj6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zj6, resolution 2.0&Aring;" /> '''Crystal structure of...)
Line 1: Line 1:
-
[[Image:1zj6.gif|left|200px]]<br />
+
[[Image:1zj6.gif|left|200px]]<br /><applet load="1zj6" size="350" color="white" frame="true" align="right" spinBox="true"
-
<applet load="1zj6" size="450" color="white" frame="true" align="right" spinBox="true"
+
caption="1zj6, resolution 2.0&Aring;" />
caption="1zj6, resolution 2.0&Aring;" />
'''Crystal structure of human ARL5'''<br />
'''Crystal structure of human ARL5'''<br />
==Overview==
==Overview==
-
ARL5 is a member of ARLs, which is widespread in high eukaryotes and, homologous between species. But no structure or biological function of, this member is reported. We expressed, purified, and resolved the, structure of human ARL5 with bound GDP3'P at 2.0 A resolution. A, comparison with the known structures of ARFs shows that besides the, typical features of ARFs, human ARL5 has specific features of its own., Bacterially expressed human ARL5 contains bound GDP3'P which is seldom, seen in other structures. The hydrophobic tail of the introduced detergent, Triton X-305 binds at the possible myristoylation site of Gly2, simulating, the myristoylated state of N-terminal amphipathic helix in vivo. The, structural features of the nucleotide binding motifs and the switch, regions prove that ARL5 will undergo the typical GDP/GTP structural cycle, as other members of ARLs, which is the basis of their biological, functions.
+
ARL5 is a member of ARLs, which is widespread in high eukaryotes and homologous between species. But no structure or biological function of this member is reported. We expressed, purified, and resolved the structure of human ARL5 with bound GDP3'P at 2.0 A resolution. A comparison with the known structures of ARFs shows that besides the typical features of ARFs, human ARL5 has specific features of its own. Bacterially expressed human ARL5 contains bound GDP3'P which is seldom seen in other structures. The hydrophobic tail of the introduced detergent Triton X-305 binds at the possible myristoylation site of Gly2, simulating the myristoylated state of N-terminal amphipathic helix in vivo. The structural features of the nucleotide binding motifs and the switch regions prove that ARL5 will undergo the typical GDP/GTP structural cycle as other members of ARLs, which is the basis of their biological functions.
==About this Structure==
==About this Structure==
-
1ZJ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and G3D as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZJ6 OCA].
+
1ZJ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=G3D:'>G3D</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJ6 OCA].
==Reference==
==Reference==
Line 14: Line 13:
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
-
[[Category: An, X.M.]]
+
[[Category: An, X M.]]
-
[[Category: Chang, W.R.]]
+
[[Category: Chang, W R.]]
-
[[Category: Liang, D.C.]]
+
[[Category: Liang, D C.]]
-
[[Category: Liu, J.F.]]
+
[[Category: Liu, J F.]]
[[Category: Shi, L.]]
[[Category: Shi, L.]]
-
[[Category: Wang, Z.X.]]
+
[[Category: Wang, Z X.]]
[[Category: G3D]]
[[Category: G3D]]
[[Category: SO4]]
[[Category: SO4]]
Line 25: Line 24:
[[Category: gtp-binding]]
[[Category: gtp-binding]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:35:18 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:08 2008''

Revision as of 14:16, 21 February 2008

Image:1zj6.gif

1zj6, resolution 2.0Å

Drag the structure with the mouse to rotate

Crystal structure of human ARL5

Overview

ARL5 is a member of ARLs, which is widespread in high eukaryotes and homologous between species. But no structure or biological function of this member is reported. We expressed, purified, and resolved the structure of human ARL5 with bound GDP3'P at 2.0 A resolution. A comparison with the known structures of ARFs shows that besides the typical features of ARFs, human ARL5 has specific features of its own. Bacterially expressed human ARL5 contains bound GDP3'P which is seldom seen in other structures. The hydrophobic tail of the introduced detergent Triton X-305 binds at the possible myristoylation site of Gly2, simulating the myristoylated state of N-terminal amphipathic helix in vivo. The structural features of the nucleotide binding motifs and the switch regions prove that ARL5 will undergo the typical GDP/GTP structural cycle as other members of ARLs, which is the basis of their biological functions.

About this Structure

1ZJ6 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.

Reference

2.0 A crystal structure of human ARL5-GDP3'P, a novel member of the small GTP-binding proteins., Wang ZX, Shi L, Liu JF, An XM, Chang WR, Liang DC, Biochem Biophys Res Commun. 2005 Jul 8;332(3):640-5. PMID:15896705

Page seeded by OCA on Thu Feb 21 16:16:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zj6"
Personal tools