1zjn
From Proteopedia
(New page: 200px<br /> <applet load="1zjn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zjn, resolution 2.61Å" /> '''Human DNA Polymeras...) |
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| - | [[Image:1zjn.gif|left|200px]]<br /> | + | [[Image:1zjn.gif|left|200px]]<br /><applet load="1zjn" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1zjn" size=" | + | |
caption="1zjn, resolution 2.61Å" /> | caption="1zjn, resolution 2.61Å" /> | ||
'''Human DNA Polymerase beta complexed with DNA containing an A-A mismatched primer terminus with dGTP'''<br /> | '''Human DNA Polymerase beta complexed with DNA containing an A-A mismatched primer terminus with dGTP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | DNA polymerases occasionally insert the wrong nucleotide. For this error | + | DNA polymerases occasionally insert the wrong nucleotide. For this error to become a mutation, the mispair must be extended. We report a structure of DNA polymerase beta (pol beta) with a DNA mismatch at the boundary of the polymerase active site. The structure of this complex indicates that the templating adenine of the mispair stacks with the primer terminus adenine while the templating (coding) cytosine is flipped out of the DNA helix. Soaking the crystals of the binary complex with dGTP resulted in crystals of a ternary substrate complex. In this case, the templating cytosine is observed within the DNA helix and forms Watson-Crick hydrogen bonds with the incoming dGTP. The adenine at the primer terminus has rotated into a syn-conformation to interact with the opposite adenine in a planar configuration. Yet, the 3'-hydroxyl on the primer terminus is out of position for efficient nucleotide insertion. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZJN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, NA and DGT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ZJN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=DGT:'>DGT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Batra, V | + | [[Category: Batra, V K.]] |
| - | [[Category: Beard, W | + | [[Category: Beard, W A.]] |
| - | [[Category: Pedersen, L | + | [[Category: Pedersen, L C.]] |
| - | [[Category: Shock, D | + | [[Category: Shock, D D.]] |
| - | [[Category: Wilson, S | + | [[Category: Wilson, S H.]] |
[[Category: DGT]] | [[Category: DGT]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: dna/lyase/transferase]] | [[Category: dna/lyase/transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:14 2008'' |
Revision as of 14:16, 21 February 2008
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Human DNA Polymerase beta complexed with DNA containing an A-A mismatched primer terminus with dGTP
Overview
DNA polymerases occasionally insert the wrong nucleotide. For this error to become a mutation, the mispair must be extended. We report a structure of DNA polymerase beta (pol beta) with a DNA mismatch at the boundary of the polymerase active site. The structure of this complex indicates that the templating adenine of the mispair stacks with the primer terminus adenine while the templating (coding) cytosine is flipped out of the DNA helix. Soaking the crystals of the binary complex with dGTP resulted in crystals of a ternary substrate complex. In this case, the templating cytosine is observed within the DNA helix and forms Watson-Crick hydrogen bonds with the incoming dGTP. The adenine at the primer terminus has rotated into a syn-conformation to interact with the opposite adenine in a planar configuration. Yet, the 3'-hydroxyl on the primer terminus is out of position for efficient nucleotide insertion.
About this Structure
1ZJN is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate., Batra VK, Beard WA, Shock DD, Pedersen LC, Wilson SH, Structure. 2005 Aug;13(8):1225-33. PMID:16084394
Page seeded by OCA on Thu Feb 21 16:16:14 2008
Categories: Homo sapiens | Single protein | Batra, V K. | Beard, W A. | Pedersen, L C. | Shock, D D. | Wilson, S H. | DGT | MG | NA | Dna/lyase/transferase
