1zlc
From Proteopedia
(New page: 200px<br /><applet load="1zlc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zlc" /> '''Solution Conformation of alpha-conotoxin PIA...) |
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| - | [[Image:1zlc.gif|left|200px]]<br /><applet load="1zlc" size=" | + | [[Image:1zlc.gif|left|200px]]<br /><applet load="1zlc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1zlc" /> | caption="1zlc" /> | ||
'''Solution Conformation of alpha-conotoxin PIA'''<br /> | '''Solution Conformation of alpha-conotoxin PIA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | alpha-Conotoxin PIA is a novel nicotinic acetylcholine receptor (nAChR) | + | alpha-Conotoxin PIA is a novel nicotinic acetylcholine receptor (nAChR) antagonist isolated from Conus purpurascens that targets nAChR subtypes containing alpha6 and alpha3 subunits. alpha-conotoxin PIA displays 75-fold higher affinity for rat alpha6/alpha3beta2beta3 nAChRs than for rat alpha3beta2 nAChRs. We have determined the three-dimensional structure of alpha-conotoxin PIA by nuclear magnetic resonance spectroscopy. The alpha-conotoxin PIA has an "omega-shaped" overall topology as other alpha4/7 subfamily conotoxins. Yet, unlike other neuronally targeted alpha4/7-conotoxins, its N-terminal tail Arg1-Asp2-Pro3 protrudes out of its main molecular body because Asp2-Pro3-Cys4-Cys5 forms a stable type I beta-turn. In addition, a kink introduced by Pro15 in the second loop of this toxin provides a distinct steric and electrostatic environment from those in alpha-conotoxins MII and GIC. By comparing the structure of alpha-conotoxin PIA with other functionally related alpha-conotoxins we suggest structural features in alpha-conotoxin PIA that may be associated with its unique receptor recognition profile. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZLC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1ZLC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLC OCA]. |
==Reference== | ==Reference== | ||
Solution structure of alpha-conotoxin PIA, a novel antagonist of alpha6 subunit containing nicotinic acetylcholine receptors., Chi SW, Lee SH, Kim DH, Kim JS, Olivera BM, McIntosh JM, Han KH, Biochem Biophys Res Commun. 2005 Dec 30;338(4):1990-7. Epub 2005 Nov 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16289101 16289101] | Solution structure of alpha-conotoxin PIA, a novel antagonist of alpha6 subunit containing nicotinic acetylcholine receptors., Chi SW, Lee SH, Kim DH, Kim JS, Olivera BM, McIntosh JM, Han KH, Biochem Biophys Res Commun. 2005 Dec 30;338(4):1990-7. Epub 2005 Nov 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16289101 16289101] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chi, S | + | [[Category: Chi, S W.]] |
| - | [[Category: Han, K | + | [[Category: Han, K H.]] |
| - | [[Category: Kim, D | + | [[Category: Kim, D H.]] |
| - | [[Category: Kim, J | + | [[Category: Kim, J S.]] |
| - | [[Category: Lee, S | + | [[Category: Lee, S H.]] |
| - | [[Category: McIntosh, J | + | [[Category: McIntosh, J M.]] |
| - | [[Category: Olivera, B | + | [[Category: Olivera, B M.]] |
[[Category: NH2]] | [[Category: NH2]] | ||
[[Category: alpha-helix]] | [[Category: alpha-helix]] | ||
| Line 25: | Line 25: | ||
[[Category: two disulfide bonds]] | [[Category: two disulfide bonds]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:40 2008'' |
Revision as of 14:16, 21 February 2008
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Solution Conformation of alpha-conotoxin PIA
Overview
alpha-Conotoxin PIA is a novel nicotinic acetylcholine receptor (nAChR) antagonist isolated from Conus purpurascens that targets nAChR subtypes containing alpha6 and alpha3 subunits. alpha-conotoxin PIA displays 75-fold higher affinity for rat alpha6/alpha3beta2beta3 nAChRs than for rat alpha3beta2 nAChRs. We have determined the three-dimensional structure of alpha-conotoxin PIA by nuclear magnetic resonance spectroscopy. The alpha-conotoxin PIA has an "omega-shaped" overall topology as other alpha4/7 subfamily conotoxins. Yet, unlike other neuronally targeted alpha4/7-conotoxins, its N-terminal tail Arg1-Asp2-Pro3 protrudes out of its main molecular body because Asp2-Pro3-Cys4-Cys5 forms a stable type I beta-turn. In addition, a kink introduced by Pro15 in the second loop of this toxin provides a distinct steric and electrostatic environment from those in alpha-conotoxins MII and GIC. By comparing the structure of alpha-conotoxin PIA with other functionally related alpha-conotoxins we suggest structural features in alpha-conotoxin PIA that may be associated with its unique receptor recognition profile.
About this Structure
1ZLC is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of alpha-conotoxin PIA, a novel antagonist of alpha6 subunit containing nicotinic acetylcholine receptors., Chi SW, Lee SH, Kim DH, Kim JS, Olivera BM, McIntosh JM, Han KH, Biochem Biophys Res Commun. 2005 Dec 30;338(4):1990-7. Epub 2005 Nov 7. PMID:16289101
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