1zld
From Proteopedia
(New page: 200px<br /><applet load="1zld" size="350" color="white" frame="true" align="right" spinBox="true" caption="1zld, resolution 1.65Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | Tan spot of wheat (Triticum aestivum), caused by the fungus Pyrenophora | + | Tan spot of wheat (Triticum aestivum), caused by the fungus Pyrenophora tritici-repentis, has significant agricultural and economic impact. Ptr ToxA (ToxA), the first discovered proteinaceous host-selective toxin, is produced by certain P. tritici-repentis races and is necessary and sufficient to cause cell death in sensitive wheat cultivars. We present here the high-resolution crystal structure of ToxA in two different crystal forms, providing four independent views of the protein. ToxA adopts a single-domain, beta-sandwich fold of novel topology. Mapping of the existing mutation data onto the structure supports the hypothesized importance of an Arg-Gly-Asp (RGD) and surrounding sequence. Its occurrence in a single, solvent-exposed loop in the protein suggests that it is directly involved in recognition events required for ToxA action. Furthermore, the ToxA structure reveals a surprising similarity with the classic mammalian RGD-containing domain, the fibronectin type III (FnIII) domain: the two topologies are related by circular permutation. The similar topologies and the positional conservation of the RGD-containing loop raises the possibility that ToxA is distantly related to mammalian FnIII proteins and that to gain entry it binds to an integrin-like receptor in the plant host. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Pyrenophora tritici-repentis]] | [[Category: Pyrenophora tritici-repentis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ciuffetti, L | + | [[Category: Ciuffetti, L M.]] |
| - | [[Category: Karplus, P | + | [[Category: Karplus, P A.]] |
| - | [[Category: Manning, V | + | [[Category: Manning, V A.]] |
| - | [[Category: Sarma, G | + | [[Category: Sarma, G N.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: beta-sandwich; rgd-motif]] | [[Category: beta-sandwich; rgd-motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:41 2008'' |
Revision as of 14:16, 21 February 2008
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Crystal structure of a RGD-containing host-selective toxin: Pyrenophora tritici-repentis Ptr ToxA
Overview
Tan spot of wheat (Triticum aestivum), caused by the fungus Pyrenophora tritici-repentis, has significant agricultural and economic impact. Ptr ToxA (ToxA), the first discovered proteinaceous host-selective toxin, is produced by certain P. tritici-repentis races and is necessary and sufficient to cause cell death in sensitive wheat cultivars. We present here the high-resolution crystal structure of ToxA in two different crystal forms, providing four independent views of the protein. ToxA adopts a single-domain, beta-sandwich fold of novel topology. Mapping of the existing mutation data onto the structure supports the hypothesized importance of an Arg-Gly-Asp (RGD) and surrounding sequence. Its occurrence in a single, solvent-exposed loop in the protein suggests that it is directly involved in recognition events required for ToxA action. Furthermore, the ToxA structure reveals a surprising similarity with the classic mammalian RGD-containing domain, the fibronectin type III (FnIII) domain: the two topologies are related by circular permutation. The similar topologies and the positional conservation of the RGD-containing loop raises the possibility that ToxA is distantly related to mammalian FnIII proteins and that to gain entry it binds to an integrin-like receptor in the plant host.
About this Structure
1ZLD is a Single protein structure of sequence from Pyrenophora tritici-repentis with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of Ptr ToxA: an RGD-containing host-selective toxin from Pyrenophora tritici-repentis., Sarma GN, Manning VA, Ciuffetti LM, Karplus PA, Plant Cell. 2005 Nov;17(11):3190-202. Epub 2005 Oct 7. PMID:16214901
Page seeded by OCA on Thu Feb 21 16:16:41 2008
