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1zmf
From Proteopedia
(New page: 200px<br /> <applet load="1zmf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zmf, resolution 1.88Å" /> '''C domain of human c...) |
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| - | [[Image:1zmf.gif|left|200px]]<br /> | + | [[Image:1zmf.gif|left|200px]]<br /><applet load="1zmf" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1zmf" size=" | + | |
caption="1zmf, resolution 1.88Å" /> | caption="1zmf, resolution 1.88Å" /> | ||
'''C domain of human cyclophilin-33(hcyp33)'''<br /> | '''C domain of human cyclophilin-33(hcyp33)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cyclophilins (CyPs) are a widespreading protein family in living organisms | + | Cyclophilins (CyPs) are a widespreading protein family in living organisms and possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase), which is inhibited by cyclosporin A (CsA). The human nuclear cyclophilin (hCyP33) is the first protein which was found to contain two RNA binding domains at the amino-terminus and a PPIase domain at the carboxyl-terminus. We isolated the hCyP33 gene from the human hematopoietic stem/progenitor cells and expressed it in Escherichia coli, and determined the crystal structure of the C domain of hCyP33 at 1.88 A resolution. The core structure is a beta-barrel covered by two alpha-helices. Superposition of the structure of the C domain of hCyP33 with the structure of CypA suggests that the C domain contains PPIase active site which binds to CsA. Furthermore, C domain seems to be able to bind with the Gag-encoded capsid (CA) of HIV-1 and may affect the viral replication of HIV-1. A key residue of the active site is changed from Ala-103-CypA to Ser-239-hCyP33, which may affect the PPIase domain/substrates interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZMF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http:// | + | 1ZMF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZMF OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 14: | ||
[[Category: Peptidylprolyl isomerase]] | [[Category: Peptidylprolyl isomerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chang, W | + | [[Category: Chang, W R.]] |
| - | [[Category: Gu, S | + | [[Category: Gu, S Y.]] |
| - | [[Category: Liang, D | + | [[Category: Liang, D C.]] |
[[Category: Wang, T.]] | [[Category: Wang, T.]] | ||
| - | [[Category: Yun, C | + | [[Category: Yun, C H.]] |
[[Category: human cyclophilin-33]] | [[Category: human cyclophilin-33]] | ||
[[Category: ppiase]] | [[Category: ppiase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:59 2008'' |
Revision as of 14:17, 21 February 2008
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C domain of human cyclophilin-33(hcyp33)
Overview
Cyclophilins (CyPs) are a widespreading protein family in living organisms and possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase), which is inhibited by cyclosporin A (CsA). The human nuclear cyclophilin (hCyP33) is the first protein which was found to contain two RNA binding domains at the amino-terminus and a PPIase domain at the carboxyl-terminus. We isolated the hCyP33 gene from the human hematopoietic stem/progenitor cells and expressed it in Escherichia coli, and determined the crystal structure of the C domain of hCyP33 at 1.88 A resolution. The core structure is a beta-barrel covered by two alpha-helices. Superposition of the structure of the C domain of hCyP33 with the structure of CypA suggests that the C domain contains PPIase active site which binds to CsA. Furthermore, C domain seems to be able to bind with the Gag-encoded capsid (CA) of HIV-1 and may affect the viral replication of HIV-1. A key residue of the active site is changed from Ala-103-CypA to Ser-239-hCyP33, which may affect the PPIase domain/substrates interactions.
About this Structure
1ZMF is a Single protein structure of sequence from Homo sapiens. Active as Peptidylprolyl isomerase, with EC number 5.2.1.8 Full crystallographic information is available from OCA.
Reference
1.88 A crystal structure of the C domain of hCyP33: a novel domain of peptidyl-prolyl cis-trans isomerase., Wang T, Yun CH, Gu SY, Chang WR, Liang DC, Biochem Biophys Res Commun. 2005 Aug 5;333(3):845-9. PMID:15963461
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