1zpq
From Proteopedia
(New page: 200px<br /><applet load="1zpq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zpq, resolution 2.8Å" /> '''STRUCTURE OF BACTERIO...) |
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| - | [[Image:1zpq.gif|left|200px]]<br /><applet load="1zpq" size=" | + | [[Image:1zpq.gif|left|200px]]<br /><applet load="1zpq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1zpq, resolution 2.8Å" /> | caption="1zpq, resolution 2.8Å" /> | ||
'''STRUCTURE OF BACTERIOPHAGE LAMBDA CII protein'''<br /> | '''STRUCTURE OF BACTERIOPHAGE LAMBDA CII protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The tetrameric cII protein from bacteriophage lambda activates | + | The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element. Here, we present the X-ray crystal structure of cII alone (2.8 A resolution) and in complex with its DNA operator from P(RE) (1.7 A resolution). The structures provide a basis for modeling of the activation complex with the RNA polymerase holoenzyme, and point to the key role for the RNA polymerase alpha subunit C-terminal domain (alphaCTD) in cII-dependent activation, which forms a bridge of protein/protein interactions between cII and the RNA polymerase sigma subunit. The model makes specific predictions for protein/protein interactions between cII and alphaCTD, and between alphaCTD and sigma, which are supported by previous genetic studies. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZPQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http:// | + | 1ZPQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZPQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Beasley, S.]] | [[Category: Beasley, S.]] | ||
| - | [[Category: Darst, S | + | [[Category: Darst, S A.]] |
| - | [[Category: Edwards, A | + | [[Category: Edwards, A M.]] |
| - | [[Category: Gussin, G | + | [[Category: Gussin, G N.]] |
[[Category: Jain, D.]] | [[Category: Jain, D.]] | ||
[[Category: Kim, Y.]] | [[Category: Kim, Y.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
| - | [[Category: Maxwell, K | + | [[Category: Maxwell, K L.]] |
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
[[Category: mcsg]] | [[Category: mcsg]] | ||
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[[Category: transcription activation]] | [[Category: transcription activation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:17:52 2008'' |
Revision as of 14:17, 21 February 2008
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STRUCTURE OF BACTERIOPHAGE LAMBDA CII protein
Overview
The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element. Here, we present the X-ray crystal structure of cII alone (2.8 A resolution) and in complex with its DNA operator from P(RE) (1.7 A resolution). The structures provide a basis for modeling of the activation complex with the RNA polymerase holoenzyme, and point to the key role for the RNA polymerase alpha subunit C-terminal domain (alphaCTD) in cII-dependent activation, which forms a bridge of protein/protein interactions between cII and the RNA polymerase sigma subunit. The model makes specific predictions for protein/protein interactions between cII and alphaCTD, and between alphaCTD and sigma, which are supported by previous genetic studies.
About this Structure
1ZPQ is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
Crystal structure of bacteriophage lambda cII and its DNA complex., Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA, Mol Cell. 2005 Jul 22;19(2):259-69. PMID:16039594
Page seeded by OCA on Thu Feb 21 16:17:52 2008
Categories: Enterobacteria phage lambda | Single protein | Beasley, S. | Darst, S A. | Edwards, A M. | Gussin, G N. | Jain, D. | Kim, Y. | MCSG, Midwest Center for Structural Genomics. | Maxwell, K L. | Helix-turn-helix | Mcsg | Midwest center for structural genomics | Protein structure initiative | Psi | Structural genomics | Transcription activation
